[巨噬细胞移动抑制因子在阻塞性睡眠呼吸暂停低通气综合征中的表达及其对内皮细胞的损伤]

[The expression of macrophage migration inhibitory factor in obstructive sleep apnea hypopnea syndrome and its injury to endothelial cells].

作者信息

Yuan P, Wang W J, Li D F, Ou Q, Gao X L

机构信息

Southern Medical University, Guangzhou 510515, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2016 Nov 12;39(11):871-875. doi: 10.3760/cma.j.issn.1001-0939.2016.11.010.

DOI:10.3760/cma.j.issn.1001-0939.2016.11.010

PMID:27852364

Abstract

To study the expression of macrophage migration inhibitory factor (MIF) in obstructive sleep apnea hypopnea syndrome (OSAHS) and its injury to endothelial cells. According to the PSG test results, subjects who were the first time to take PSG examination without treatment (=71) were divided into a control group (=20), a mild OSAHS group (=19), a moderate OSAHS group (=15) and severe OSAHS group (=17). For each patients, 4 ml fasting peripheral blood was obtained when PSG was finished around 6: 30 in the next morning, and the MIF level in plasma was detected with the ELISA method. Peripheral blood mononuclear cells (PBMC) from the control group and the severe OSAHS patients were cocultured with umbilical vein endothelial cells (HUVEC) for 72 hours. The apoptosis of HUVEC was detected by flow cytometry, while ET-1, NO, sICAM-1 and IL-6 in the supernatants were measured with the ELISA method. The plasma level of MIF in the control group and the mild, the moderate, and the severe OSAHS patients was (26±8), (28±9), (31±14), (39±15) ng/ml, respectively (=15.65, <0.001), and it was higher in the severe OSAHS group as compared to the control group(<0.01). The level of MIF was associated positively with the apnea hypoventilation index (AHI, =0.365, =0.008) and the oxygen index reduction (ODI, =0.308, =0.308) n but negatively with the lowest blood oxygen (=0.323, =0.323). Endothelial cell apoptosis rate in the control group and the severe OSAHS group was (2.94±1.02) %, (8.23± 3.01) %, respectively, =5.97, <0.001. ET-1 in the control group and the severe OSAHS group was (6.71±5.52), (9.88±4.79) pg/ml, respectively, =3.018, =0.141. sICAM-1 in the control group and the severe OSAHS group was (11±8), (20±7) ng/ml, respectively, =7.58, =0.014. NO in the control group and the severe OSAHS group was (35±16), (25±5) mol/L, respectively, =2.01, =0.067. IL-6 in the control group and the severe OSAHS group was (220±42), (436±178) mol/L, respectively, =2.77, <0.05. MIF is closely related to the degree of OSAHS severity, and it may be involved in the development and endothelial injury in OSAHS.

摘要

研究巨噬细胞移动抑制因子（MIF）在阻塞性睡眠呼吸暂停低通气综合征（OSAHS）中的表达及其对内皮细胞的损伤。根据多导睡眠图（PSG）测试结果，首次接受未经治疗的PSG检查的受试者（n = 71）被分为对照组（n = 20）、轻度OSAHS组（n = 19）、中度OSAHS组（n = 15）和重度OSAHS组（n = 17）。对于每位患者，在次日早晨6:30左右PSG检查结束时采集4 ml空腹外周血，采用酶联免疫吸附测定（ELISA）法检测血浆中的MIF水平。将对照组和重度OSAHS患者的外周血单个核细胞（PBMC）与脐静脉内皮细胞（HUVEC）共培养72小时。采用流式细胞术检测HUVEC的凋亡情况，同时采用ELISA法检测上清液中的内皮素-1（ET-1）、一氧化氮（NO）、可溶性细胞间黏附分子-1（sICAM-1）和白细胞介素-6（IL-6）。对照组、轻度、中度和重度OSAHS患者血浆中MIF水平分别为（26±8）、（28±9）、（31±14）、（39±15）ng/ml（F = 15.65，P < 0.001），重度OSAHS组高于对照组（P < 0.01）。MIF水平与呼吸暂停低通气指数（AHI，r = 0.365，P = 0.008）和氧减指数（ODI，r = 0.308，P = 0.008）呈正相关，与最低血氧饱和度（r = -0.323，P = 0.032）呈负相关。对照组和重度OSAHS组内皮细胞凋亡率分别为（2.94±1.02）%、（8.23±3.01）%（t = 5.97，P < 0.001）。对照组和重度OSAHS组ET-1分别为（6.71±5.52）、（9.88±4.79）pg/ml（t = 3.018，P = 0.014）。对照组和重度OSAHS组sICAM-1分别为（11±8）、（20±7）ng/ml（t = 7.58，P = 0.014）。对照组和重度OSAHS组NO分别为（35±16）、（25±5）μmol/L（t = 2.01，P = 0.067）。对照组和重度OSAHS组IL-6分别为（220±42）、（436±178）μmol/L（t = 2.77，P < 0.05）。MIF与OSAHS严重程度密切相关，可能参与OSAHS的发生发展及内皮损伤。