亚硫酸氢盐转化后的DNA文库的Illumina测序
Illumina Sequencing of Bisulfite-Converted DNA Libraries.
作者信息
Lizardi Paul M, Yan Qin, Wajapeyee Narendra
出版信息
Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot094870. doi: 10.1101/pdb.prot094870.
PMID:27852841
Abstract
Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.
摘要
在此，我们描述了一种在Illumina Genome Analyzer II平台上使用单端测序的标准甲基化DNA免疫共沉淀测序（MethylC-seq）方案。该方案包括将甲基化测序接头连接到经超声处理的基因组DNA上、凝胶纯化、亚硫酸氢钠转化、聚合酶链反应（PCR）扩增及测序。
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