Calise F, Capron-Laudereau M, Gugenheim J, Pin M L, Gigou M, Cuomo O, Reynes M, Bismuth H
Département de Chirurgie Expérimentale, Hôpital Paul Brousse, Villejuif, France.
Eur Surg Res. 1989;21(1):18-24. doi: 10.1159/000128999.
The risk of overwhelming infections is greatly increased after splenectomy. In this experimental study in rats, we investigated whether the administration of fibronectinrich cryoprecipitate can improve the survival rate of splenectomized autotransplanted rats subjected to an intravenous challenge with endotoxin. Inbred Lewis rats were divided into four groups: A, splenectomy; B, splenectomy + splenic autotransplantation; C, splenectomy, splenic autotransplantation + fibronectin treatment, and D, sham. Five months after surgery, rats were challenged intravenously with Escherichia coli endotoxin. Immunoglobulin (IgG, IgM, IgA), complement and fibronectin levels were measured before surgery and endotoxin challenge, and 48 h after endotoxin challenge. The survival rate of splenectomized rats was not significantly improved by autotransplantation of splenic tissue, but was significantly (p less than 0.05) improved by autotransplantation and fibronectin treatment. The levels of fibronectin, immunoglobulins and/or complements were significantly decreased after endotoxin challenge in control and in autotransplanted fibronectin-treated rats. The survival improvement of autotransplanted rats treated by fibronectin is probably due to increased endotoxin phagocytosis and clearance.
脾切除术后严重感染的风险会大大增加。在这项针对大鼠的实验研究中，我们调查了给予富含纤维连接蛋白的冷沉淀物是否能提高接受内毒素静脉注射攻击的脾切除自体移植大鼠的存活率。近交系Lewis大鼠被分为四组：A组，脾切除术；B组，脾切除术 + 脾自体移植术；C组，脾切除术、脾自体移植术 + 纤维连接蛋白治疗，以及D组，假手术组。术后五个月，大鼠接受大肠杆菌内毒素静脉注射攻击。在手术前、内毒素攻击前以及内毒素攻击后48小时测量免疫球蛋白（IgG、IgM、IgA）、补体和纤维连接蛋白水平。脾切除大鼠的存活率并未因脾组织自体移植而显著提高，但自体移植和纤维连接蛋白治疗使其存活率显著提高（p < 0.05）。在内毒素攻击后，对照组和接受纤维连接蛋白治疗的自体移植大鼠的纤维连接蛋白、免疫球蛋白和/或补体水平显著降低。纤维连接蛋白治疗的自体移植大鼠存活率提高可能是由于内毒素吞噬和清除增加。
