Sun Yuewen, Qiu Hanchen, Ou Mingchun, Chen Runli, Liang Gang
Department of Pharmacy, Guangxi Medical University, Nanning, Guangxi, China.
J Int Med Res. 2016 Dec;44(6):1395-1402. doi: 10.1177/0300060516672368. Epub 2016 Nov 18.
Objective Taccaoside, a steroidal saponin, has been shown to be cytotoxic, although the mechanism of cytotoxicity remains unclear. This study examined the effect of taccaoside on the human hepatocellular carcinoma (HCC) cell lines SMMC-7721 and Bel-7404. Methods The antiproliferative effect of taccaoside were measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Cells were stained with Hoechst 33258 to observe morphology. Cell cycle and apoptosis were analysed by flow cytometry. Caspase activation was detected using specific assays, and PARP, Bax and Bcl-2 expression were analysed using western blotting. Results Taccaoside showed antiproliferative effect on HCC cell lines growth in a concentration- and time-dependent manner. Taccaoside arrested cell cycle in the G2/M phase and induced caspase-dependent apoptosis. Western blotting indicated that taccaoside upregulated Bax expression and downregulated Bcl-2 expression. PARP cleavage was observed following taccaoside treatment. Conclusions This study showed that taccaoside may inhibit HCC cell proliferation by inducing apoptosis.
目的 薯蓣皂苷元是一种甾体皂苷，已被证明具有细胞毒性，但其细胞毒性机制尚不清楚。本研究检测了薯蓣皂苷元对人肝癌细胞系SMMC - 7721和Bel - 7404的作用。方法 采用3-(4,5 - 二甲基噻唑 - 2 - 基)-2,5 - 二苯基四氮唑溴盐法检测薯蓣皂苷元的抗增殖作用。用Hoechst 33258对细胞进行染色以观察形态。通过流式细胞术分析细胞周期和凋亡。使用特异性检测方法检测半胱天冬酶激活情况，并通过蛋白质印迹法分析聚（ADP - 核糖）聚合酶（PARP）、Bax和Bcl - 2的表达。结果 薯蓣皂苷元对肝癌细胞系的生长具有抗增殖作用，且呈浓度和时间依赖性。薯蓣皂苷元使细胞周期停滞于G2/M期并诱导半胱天冬酶依赖性凋亡。蛋白质印迹法表明，薯蓣皂苷元上调Bax表达并下调Bcl - 2表达。薯蓣皂苷元处理后观察到PARP裂解。结论 本研究表明，薯蓣皂苷元可能通过诱导凋亡来抑制肝癌细胞增殖。
