Yu Juan, Yang Li-Nan, Wu Yan-Yun, Li Bao-Hua, Weng Sheng-Mei, Hu Chun-Lan, Han Yong-Ling
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
Department of Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province, China.
Neural Regen Res. 2016 Sep;11(9):1431-1437. doi: 10.4103/1673-5374.191216.
13-Methyltetradecanoic acid can stabilize cell membrane and have anti-inflammatory, antioxidant and anti-apoptotic effects. Previous studies mainly focused on peripheral nerve injury, but seldom on the central nervous system. We investigated whether these properties of 13-methyltetradecanoic acid have a neuroprotective effect on focal cerebral ischemia/reperfusion injury, and detected the expression of basic fibroblast growth factor and vascular endothelial growth factor. This study established rat models of middle cerebral artery occlusion/reperfusion injury by ischemia for 2 hours and reperfusion for 24 hours. At the beginning of reperfusion, 13-methyltetradecanoic acid 10, 40 or 80 mg/kg was injected into the tail vein. Results found that various doses of 13-methyltetradecanoic acid effectively reduced infarct volume, mitigate cerebral edema, and increased the mRNA and protein expression of basic fibroblast growth factor and vascular endothelial growth factor at 24 hours of reperfusion. The effect was most significant in the 13-methyltetradecanoic acid 40 and 80 mg/kg groups. The findings suggest that 13-methyltetradecanoic acid can relieve focal ischemia/reperfusion injury immediately after reperfusion, stimulate the upregulation of basic fibroblast growth factor and vascular endothelial growth factor to exert neuroprotective effects.
13-甲基十四烷酸可稳定细胞膜，具有抗炎、抗氧化和抗凋亡作用。以往研究主要集中在外周神经损伤，而对中枢神经系统研究较少。我们研究了13-甲基十四烷酸的这些特性对局灶性脑缺血/再灌注损伤是否具有神经保护作用，并检测了碱性成纤维细胞生长因子和血管内皮生长因子的表达。本研究通过缺血2小时和再灌注24小时建立大鼠大脑中动脉闭塞/再灌注损伤模型。在再灌注开始时，将10、40或80mg/kg的13-甲基十四烷酸注入尾静脉。结果发现，不同剂量的13-甲基十四烷酸均能有效减少梗死体积，减轻脑水肿，并在再灌注24小时时增加碱性成纤维细胞生长因子和血管内皮生长因子的mRNA和蛋白表达。在13-甲基十四烷酸40mg/kg和80mg/kg组中效果最为显著。研究结果表明，13-甲基十四烷酸可在再灌注后立即减轻局灶性缺血/再灌注损伤，刺激碱性成纤维细胞生长因子和血管内皮生长因子上调以发挥神经保护作用。
