Animal Science Department, College of Agriculture and Animal Husbandry, Qinghai University, Ning Da Road 251, Xining 810016, China.
Molecules. 2023 Oct 23;28(20):7222. doi: 10.3390/molecules28207222.
Branched-chain fatty acids (BCFAs) are natural components with a variety of biological activities. However, the regulation of lipid metabolism by BCFAs is unknown. It was dedicated to examining the impacts of BCFAs inferred from yak ghee on the expression of qualities related to lipid metabolism, natural pathways, and intestinal microbiota in mice. The treatment group (purified BCFAs from yak ghee) exhibited a decrease in cholesterol levels; a decrease in levels; downregulation of , , , , , , , , , and gene expression; and upregulation of , , , , and gene expression. Gut microbiota 16S rDNA sequencing analysis showed that the treatment group improved the gut microbiota by increasing the relative abundances and increasing the short-chain fatty acid levels produced by the genera , , , , , and . After adding BCFAs to cultured breast cancer cells, pathways that were downregulated were found to be related to fatty acid degradation and fatty acid metabolism, while 20 other pathways were upregulated. Our results suggest that BCFAs reduce body fat in mice by modulating intestinal flora and lipid metabolism and modulating fatty acid metabolism in breast cancer cells.
支链脂肪酸（BCFAs）是具有多种生物学活性的天然成分。然而，BCFAs 对脂代谢的调节作用尚不清楚。本研究致力于探讨从牦牛酥油中推断出的 BCFAs 对小鼠与脂代谢、天然通路和肠道微生物群相关特性表达的影响。实验组（牦牛酥油中的纯化 BCFAs）表现出胆固醇水平降低； 水平降低；下调 、 、 、 、 、 、 、 和 基因表达；和上调 、 、 、 和 基因表达。肠道微生物群 16S rDNA 测序分析表明，实验组通过增加相对丰度和增加属 、 、 、 、 和 产生的短链脂肪酸水平来改善肠道微生物群。在向培养的乳腺癌细胞中添加 BCFAs 后，发现下调的途径与脂肪酸降解和脂肪酸代谢有关，而其他 20 个途径则上调。我们的结果表明，BCFAs 通过调节肠道菌群和脂代谢以及调节乳腺癌细胞中的脂肪酸代谢来减少小鼠体内脂肪。
