Hayakawa M, Masuda T, Higa I, Koyama Y, Hatano T, Oda M, Osawa A
Nihon Hinyokika Gakkai Zasshi. 1989 Jan;80(1):28-34. doi: 10.5980/jpnjurol1989.80.28.
We herein report the preliminary but appreciable results of regional transarterial infusion of 2 gravity subtypes of autologous lymphokine-activated killer (LAK) cells into the metastatic sites in combination with systemic recombinant interleukin-2 (rIL-2) administration in 3 patients with advanced renal cell carcinoma. Leukapheresis was performed once a week and peripheral blood lymphocytes were separated into 2 different subtypes by Percoll gradient centrifugation. These lymphocytes were incubated with rIL-2 for a few days to induce LAK cells. LAK cells were transferred to the metastatic lesions through cannula twice a week. A large iliac bone metastasis disappeared 3 months after the initial LAK cell therapy via a superior gluteal artery. A case of complete disappearance of psoas muscle and para-aortic lymphnode metastasis as well as partial regression of a lumbar bone metastasis was seen after lumbar arterial infusion treatment. Another case with brain metastasis showed a rapid exacerbation of brain edema after one week's LAK therapy. Our treatment modality seems to be worthwhile and promising for treatment of the advanced renal cell carcinoma.
我们在此报告3例晚期肾细胞癌患者的初步但可观的结果，即通过经动脉向转移部位局部输注2种重力亚型的自体淋巴因子激活的杀伤（LAK）细胞，并联合全身给予重组白细胞介素-2（rIL-2）。每周进行一次白细胞分离术，通过Percoll梯度离心将外周血淋巴细胞分离为2种不同亚型。这些淋巴细胞与rIL-2孵育数天以诱导LAK细胞。LAK细胞每周通过插管转移至转移病灶两次。在最初通过臀上动脉进行LAK细胞治疗3个月后，一处大的髂骨转移灶消失。经腰动脉灌注治疗后，1例腰大肌和腹主动脉旁淋巴结转移灶完全消失，腰椎骨转移灶部分消退。另一例脑转移患者在接受一周的LAK治疗后，脑水肿迅速加重。我们的治疗方式对于晚期肾细胞癌的治疗似乎是值得的且有前景的。
