Mäkelä M J, Smith R H, Lund G A, Salmi A A
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Scand J Immunol. 1989 May;29(5):597-607. doi: 10.1111/j.1365-3083.1989.tb01163.x.
BALB/c mice were pretreated with cyclophosphamide and immunized 2 days later with inactivated, purified measles virus (MV) mixed with dimethyl dioctadecyl ammonium bromide (DDA). Seven days later, lymph nodes (LN) were removed and lymphocytes cultured in the presence of purified MV antigens. MV haemagglutinin (H) was found to be a major antigen responsible for proliferation of the lymphocytes. Incorporation of purified H into liposomes significantly enhanced the proliferative response compared with purified H alone. Response to MV nucleocapsid protein was only moderate, and insertion of this protein into liposomes did not improve the response. As an attempt to analyse T-cell epitopes of MV H, three synthetic peptides previously found to elicit a strong antibody response were used both as priming and stimulating antigens. None of the peptides was able to elicit a secondary response when MV-primed LN cells were stimulated in vitro. However, each peptide primed T cells for a secondary challenge with purified, inactivated MV, which was demonstrated by proliferation and a delayed-type hypersensitivity assay and also by transfer experiments with peptide-primed cells.
将BALB/c小鼠用环磷酰胺进行预处理，2天后用与二甲基二十八烷基溴化铵（DDA）混合的灭活纯化麻疹病毒（MV）进行免疫。7天后，取出淋巴结（LN），并在纯化的MV抗原存在的情况下培养淋巴细胞。发现MV血凝素（H）是负责淋巴细胞增殖的主要抗原。与单独的纯化H相比，将纯化的H掺入脂质体中显著增强了增殖反应。对MV核衣壳蛋白的反应仅为中等程度，并且将该蛋白插入脂质体中并未改善反应。作为分析MV H的T细胞表位的尝试，使用先前发现能引发强烈抗体反应的三种合成肽作为引发和刺激抗原。当在体外刺激经MV引发的LN细胞时，没有一种肽能够引发二次反应。然而，每种肽都能使T细胞对纯化的灭活MV进行二次攻击致敏，这通过增殖和迟发型超敏反应测定以及用肽引发细胞的转移实验得到了证实。
