Kong Dexian, Hu Fangdong, Jiang Lili, Lin Jiandi, Wei Qiaohua, Chi Yuwu, Chen Guonan
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety and College of Chemistry, Fuzhou University, Fujian, China.
Luminescence. 2017 Aug;32(5):745-750. doi: 10.1002/bio.3245. Epub 2016 Nov 15.
Based on the strong enhancement effect of procaterol hydrochloride on the electrochemiluminescence (ECL) of Ru(bpy) (bpy = 2,2'-bipyridine) in an alkaline H PO -NaOH buffer solution on a bare Pt electrode, a simple, rapid and sensitive method was developed for the determination of procaterol hydrochloride. The optimum conditions for the enhanced ECL have been developed in detail in this work. Under optimum conditions, the logarithmic ECL enhancement vs. the logarithmic concentration of procaterol hydrochloride is linear over a wide concentration range of 2.0 × 10 to 2.0 × 10 M (r = 0.9976), with a limit of detection of 1.1 × 10 M (S/N = 3), and a relative standard deviation of 2.1% (n = 7, c = 5.0 × 10 M). The proposed method was applied to the determination of this drug in tablets with recoveries of 89.7%-98.5%. In addition, a possible mechanism for the enhanced ECL of Ru(bpy) , which is caused by ProH, has also been proposed.
基于盐酸丙卡特罗在碱性H₃PO₄-NaOH缓冲溶液中对裸铂电极上Ru(bpy)₃²⁺（bpy = 2,2'-联吡啶）的电化学发光（ECL）具有强烈增强作用，建立了一种简单、快速且灵敏的测定盐酸丙卡特罗的方法。本工作详细研究了增强ECL的最佳条件。在最佳条件下，盐酸丙卡特罗对数浓度与对数ECL增强值在2.0×10⁻⁹至2.0×10⁻⁵ M的宽浓度范围内呈线性关系（r = 0.9976），检测限为1.1×10⁻⁹ M（S/N = 3），相对标准偏差为2.1%（n = 7，c = 5.0×10⁻⁶ M）。该方法用于片剂中该药物的测定，回收率为89.7% - 98.5%。此外，还提出了由ProH引起的Ru(bpy)₃²⁺ ECL增强的可能机制。
Zotero 插件