Sharma Dhara, Goel Harish Chandra, Chauhan Sonal
Dhara Sharma, Amity Center for Radiation Biology, Amity University, Sector-125, Noida-201303, U.P., India.
Appl Physiol Nutr Metab. 2016 Dec;41(12):1248-1254. doi: 10.1139/apnm-2016-0136.
The cucurbits (prebiotics) were investigated as novel agents for radio-modification against gastrointestinal injury. The cell-cycle fractions and DNA damage were monitored in HCT-15 cells. A cucurbit extract was added to culture medium 2 h before irradiation (6 Gy) and was substituted by fresh medium at 4 h post-irradiation. The whole extract of the fruits of Lagenaria siceraria, Luffa cylindrica, or Cucurbita pepo extract enhanced G fractions (42%, 34%, and 37%, respectively) as compared with control (20%) and irradiated control (31%). With cucurbits, the comet tail length remained shorter (L. siceraria, 28 μm; L. cylindrica, 34.2 μm; C. pepo, 36.75 μm) than irradiated control (41.75 μm). For in vivo studies, L. siceraria extract (2 mg/kg body weight) was administered orally to mice at 2 h before and 4 and 24 h after whole-body irradiation (10 Gy). L. siceraria treatment restored the glutathione contents to 48.8 μmol/gm as compared with control (27.6 μmol/gm) and irradiated control (19.6 μmol/gm). Irradiation reduced the villi height from 379 to 350 μm and width from 54 to 27 μm. L. siceraria administration countered the radiation effects (length, 366 μm; width, 30 μm, respectively) and improved the villi morphology and tight junction integrity. This study reveals the therapeutic potential of cucurbits against radiation-induced gastrointestinal injury.
葫芦科植物（益生元）作为新型放射修饰剂用于对抗胃肠道损伤的研究。在HCT - 15细胞中监测细胞周期分数和DNA损伤情况。在照射（6 Gy）前2小时将葫芦科植物提取物添加到培养基中，并在照射后4小时用新鲜培养基替换。与对照组（20%）和照射对照组（31%）相比，丝瓜、圆筒丝瓜或西葫芦果实的全提取物分别使G期分数提高（分别为42%、34%和37%）。使用葫芦科植物后，彗星尾长仍然较短（丝瓜为28μm；圆筒丝瓜为34.2μm；西葫芦为36.75μm），而照射对照组为41.75μm。在体内研究中，在全身照射（10 Gy）前2小时以及照射后4小时和24小时，给小鼠口服丝瓜提取物（2 mg/kg体重）。与对照组（27.6μmol/g）和照射对照组（19.6μmol/g）相比，丝瓜处理使谷胱甘肽含量恢复到48.8μmol/g。照射使绒毛高度从379μm降低到350μm，宽度从54μm降低到27μm。施用丝瓜可对抗辐射效应（长度分别为366μm；宽度分别为30μm），并改善绒毛形态和紧密连接完整性。这项研究揭示了葫芦科植物对辐射诱导的胃肠道损伤的治疗潜力。
