中国北方牛泰勒虫病血清流行率
Seroprevalence of bovine theileriosis in northern China.
作者信息
Li Yaqiong, Liu Zhijie, Liu Junlong, Yang Jifei, Li Qian, Guo Pengfei, Guan Guiquan, Liu Guangyuan, Luo Jianxun, Yin Hong, Li Youquan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, People's Republic of China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
出版信息
Parasit Vectors. 2016 Nov 18;9(1):591. doi: 10.1186/s13071-016-1882-x.
BACKGROUND
Bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. Here, an indirect enzyme-linked immunosorbent assay (iELISA) based on Theileria luwenshuni surface protein (TlSP) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern China.
METHODS
We used the BugBuster Ni-NTA His•Bind Purification Kit to purify recombinant TlSP (rTlSP), which was subsequently analyzed by Western Blotting to evaluate cross-reactivity with other pathogen-positive sera. The iELISA method based on rTlSP was successfully developed. Sera from 2005 blood samples were tested with the rTlSP-iELISA method, and blood smears from these samples were observed by microscopy.
RESULTS
The specificity of iELISA was 98.9%, the sensitivity was 98.5%, and the cut-off was selected as 24.6%. Western Blot analysis of rTlSP confirmed that there were cross-reactions with Theileria luwenshuni, Theileria uilenbergi, Theileria ovis, Theileria annulata, Theileria orientalis and Theileria sinensis. The epidemiological survey showed that the highest positive rate of bovine theileriosis was 98.3%, the lowest rate was 84.1%, and the average positive rate was 95.4% by iELISA. With microscopy, the highest positive rate was 38.9%, the lowest rate was 5.1%, and the relative average positive rate was 13.7%.
CONCLUSIONS
An rTlSP-iELISA was developed to detect circulating antibodies against bovine Theileria in northern China. This is the first report on the seroprevalence of bovine theileriosis in northern China, and it also provides seroepidemiological data on bovine theileriosis in China.
背景
牛泰勒虫病是一种由蜱传播的常见疾病，可在全球范围内导致肉牛和奶牛的损失。在此，基于鲁氏泰勒虫表面蛋白（TlSP）开发了一种间接酶联免疫吸附测定（iELISA），并用于在中国北方开展牛泰勒虫病的血清流行病学调查。
方法
我们使用BugBuster Ni-NTA His•Bind纯化试剂盒纯化重组TlSP（rTlSP），随后通过蛋白质免疫印迹分析评估其与其他病原体阳性血清的交叉反应性。成功开发了基于rTlSP的iELISA方法。用rTlSP-iELISA方法检测了2005份血样的血清，并通过显微镜观察这些血样的血涂片。
结果
iELISA的特异性为98.9%，敏感性为98.5%，截断值选定为24.6%。rTlSP的蛋白质免疫印迹分析证实，其与鲁氏泰勒虫、乌氏泰勒虫、绵羊泰勒虫、环形泰勒虫、东方泰勒虫和中华泰勒虫存在交叉反应。流行病学调查显示，通过iELISA检测，牛泰勒虫病的最高阳性率为98.3%，最低阳性率为84.1%，平均阳性率为95.4%。通过显微镜观察，最高阳性率为38.9%，最低阳性率为5.1%，相对平均阳性率为13.7%。
结论
开发了一种rTlSP-iELISA来检测中国北方牛泰勒虫循环抗体。这是中国北方牛泰勒虫病血清流行率的首次报告，也为中国牛泰勒虫病提供了血清流行病学数据。
Zotero 插件