College of Animal Science, Tarim University, Tarim Road 1487, Alar, Xinjiang 843300, PR China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Wenhua Road 95, Zhengzhou, Henan 450002, PR China.
Ticks Tick Borne Dis. 2018 Sep;9(6):1446-1450. doi: 10.1016/j.ttbdis.2018.06.017. Epub 2018 Jun 30.
Bovine theileriosis is a common tick-born disease infected by Theileria spp. causing loss of beef and dairy cattle worldwide, therefore, the aim of this study was to determine the prevalence and genetic diversity of Theileria spp. in Southern Xinjiang Uyghur Autonomous Region, China. We screened 493 dairy cattle blood samples from Southern Xinjiang to detect Theileria spp. by PCR. The overall prevalence of Theileria spp. was 23.5% (116/493). The most frequent Theileria sp. was Theileria annulata 22.5% (111/493), followed by T. orientalis (0.6%, 3/493) and T. ovis (0.2%, 1/493). Additionally, one sample was co-infected with T. annulata and T. orientalis. Phylogenetic analysis showed that the 11 T. annulata Tams1 sequences grouped into three sequence clusters that belonged to group 2 (Asia group) and shared 96.4% to 99.9% identity with to each other. Furthermore, our results revealed the presence of several T. orientalis MPSP genotypes (types 1, 2, and 5) in this region. In this study, T. annulata was found to be the most prevalent Theileria species in dairy cattle from Southern Xinjiang. Theileria ovis was detected in one DNA sample of a free-grazing dairy cow from China for the first time.
牛泰勒虫病是一种常见的蜱传疾病，由泰勒虫属引起，导致全球肉牛和奶牛损失，因此，本研究旨在确定中国新疆南部地区泰勒虫属的流行情况和遗传多样性。我们通过 PCR 从新疆南部筛选了 493 份奶牛血液样本，以检测泰勒虫属。泰勒虫属的总流行率为 23.5%（116/493）。最常见的泰勒虫种是环形泰勒虫 22.5%（111/493），其次是东方泰勒虫（0.6%，3/493）和绵羊泰勒虫（0.2%，1/493）。此外，有一个样本同时感染了环形泰勒虫和东方泰勒虫。系统发育分析表明，11 个环形泰勒虫 Tams1 序列分为三个序列群，属于第 2 组（亚洲组），彼此之间的同源性为 96.4%至 99.9%。此外，我们的结果表明，该地区存在几种东方泰勒虫 MPSP 基因型（类型 1、2 和 5）。在本研究中，环形泰勒虫是新疆南部奶牛中最常见的泰勒虫种。绵羊泰勒虫首次在来自中国的自由放牧奶牛的一个 DNA 样本中被检测到。
