Hale Amanda R, Ross Ann H
Department of Biological Sciences, NCSU, 127 David Clark Labs, Campus Box 7617, Raleigh, NC, 27695-7617.
Forensic Sciences Institute, NCSU, Raleigh, NC, 27695.
J Forensic Sci. 2017 Mar;62(2):399-404. doi: 10.1111/1556-4029.13273. Epub 2016 Nov 16.
It is common for researchers using animal or human remains for scientific study to freeze samples prior to use. However, effects of freezing on bone macro- or microstructure are relatively unknown. The research objective of this study was to determine whether freezing could potentially bias experimental results by analyzing changes in bone mineral density (BMD) with the freezing of remains over time. Eight fetal pigs were scanned to determine their initial BMD before freezing. Three piglets underwent a freeze-thaw cycle to assess the effects of the freezing process. Four piglets were frozen and scanned weekly for 20 weeks to assess freezing over time. The overall average between the fresh initial scan and final frozen scan was significantly different (p < 0.001). Per contra, the final thawed BMD scans did not differ from the initial fresh scan (p = 0.418). Thus, completely thawed remains are recommended for experimental studies.
对于使用动物或人类遗体进行科学研究的研究人员来说，在使用前冷冻样本是很常见的。然而，冷冻对骨骼宏观或微观结构的影响相对未知。本研究的目的是通过分析随着遗体冷冻时间的推移骨密度（BMD）的变化，来确定冷冻是否可能使实验结果产生偏差。对八只胎猪进行扫描以确定其冷冻前的初始骨密度。三只仔猪经历了冻融循环以评估冷冻过程的影响。四只仔猪被冷冻，并在20周内每周进行扫描以评估随时间的冷冻情况。新鲜初始扫描和最终冷冻扫描之间的总体平均值存在显著差异（p < 0.001）。相反，最终解冻后的骨密度扫描与初始新鲜扫描没有差异（p = 0.418）。因此，建议在实验研究中使用完全解冻的遗体。
