Ben Othman Sana, Katsuno Nakako, Kitayama Akemi, Fujimura Makoto, Kitaguchi Kohji, Yabe Tomio
United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1132, Japan.
Shinsei Co. Ltd., 29 Katabashinmachi, Kitanagoya, Aichi 481-8526, Japan.
Nutr Res. 2016 Oct;36(10):1130-1139. doi: 10.1016/j.nutres.2016.07.007. Epub 2016 Jul 28.
Defatted sesame seed flour is recovered as a byproduct after oil extraction and is usually considered a waste product. Previously, we showed that water-soluble fractions purified from defatted white and gold sesame seed flour exhibited good antioxidant activity in vitro. We also identified ferulic acid and vanillic acid as the bioactive antioxidants in both white and gold sesame seed water-soluble fractions (WS-wsf and GS-wsf, respectively). In this study, we hypothesized that WS-wsf and GS-wsf may have neuroprotective effects due to their antioxidant potential. Treatment with WS-wsf for 24 hours enhanced human neuroblastoma SH-SY5Y cell viability and proliferation, while GS-wsf, ferulic acid, and vanillic acid did not show similar effects. In addition, WS-wsf (1-3 mg/mL) significantly and dose-dependently protected SH-SY5Y cells against camptothecin-induced apoptosis, suggesting the involvement of an anti-apoptosis mechanism in the neuroprotective effects of WS-wsf. In fact, treatment with WS-wsf significantly decreased the mRNA expression levels of pro-apoptotic Bax and p53 genes. WS-wsf also enhanced Bcl-2 protein level and Akt phosphorylation. Taken together, this study showed that WS-wsf has interesting neuroprotective potential via an anti-apoptotic mechanism, which is independent from its antioxidant capacity.
脱脂芝麻粉是油脂提取后的副产品，通常被视为废品。此前，我们发现从脱脂白芝麻粉和黄金芝麻粉中纯化得到的水溶性组分在体外表现出良好的抗氧化活性。我们还确定阿魏酸和香草酸分别是白芝麻和黄金芝麻水溶性组分（分别为WS-wsf和GS-wsf）中的生物活性抗氧化剂。在本研究中，我们假设WS-wsf和GS-wsf可能因其抗氧化潜力而具有神经保护作用。用WS-wsf处理24小时可提高人神经母细胞瘤SH-SY5Y细胞的活力和增殖能力，而GS-wsf、阿魏酸和香草酸则未表现出类似效果。此外，WS-wsf（1-3 mg/mL）显著且剂量依赖性依赖性地地保护SH-SY5Y细胞免受喜树碱诱导的凋亡，表明抗凋亡机制参与了WS-wsf的神经保护作用。事实上，用WS-wsf处理可显著降低促凋亡基因Bax和p53的mRNA表达水平。WS-wsf还提高了Bcl-2蛋白水平并增强了Akt磷酸化。综上所述，本研究表明WS-wsf通过抗凋亡机制具有有趣的神经保护潜力，这与其抗氧化能力无关。
