Kindt Nadège, Descamps Géraldine, Seminerio Imelda, Bellier Justine, Lechien Jérôme R, Pottier Charles, Larsimont Denis, Journé Fabrice, Delvenne Philippe, Saussez Sven
Laboratory of Anatomy and Cellular Biology, Research Institute for Health Sciences and Technology, Faculty of Medicine and Pharmacy, University of Mons, 7000 Mons, Belgium.
Department of Pathology, C.H.U. - SART TILMAN, University of Liège, 4000 Liège, Belgium.
Oral Oncol. 2016 Nov;62:1-10. doi: 10.1016/j.oraloncology.2016.08.016. Epub 2016 Sep 13.
Head and neck squamous cell carcinomas (HNSCCs) exhibit great biological heterogeneity and relatively poor prognosis. Tobacco and alcohol consumption is involved in the cause of the majority of these cancers, but over the last several years, Human Papilloma Virus (HPV) infection has increased specifically in oropharyngeal cancers and become an additional risk factor. Here, we evaluated the number of Langerhans cells (LCs) in HNSCC and reporting its prognostic power in comparison to other risk factors.
Our clinical series was composed of 25 tumor-free peritumoral epithelium, 64 low-grade dysplasia, 54 high-grade dysplasia and 125 carcinoma samples. HPV was detected by E6/E7 qPCR and p16 immunohistochemistry. CD1a-positive LCs were counted in intra-tumoral and stromal compartments as well as lymph nodes. MIP-3α was assessed in carcinomas using immunohistochemistry.
Univariate Cox regression analyses demonstrated that high LC number is associated with longer recurrence-free survival in both intra-tumoral and stromal compartments and longer overall survival in stromal compartment. Tobacco and alcohol habits, but not HPV status, are also correlated with poor prognoses in terms of recurrence. Multivariate analyses reported stromal LC number as a strong prognostic factor independent of tobacco, alcohol and HPV status. Moreover, LC number is higher in tumors and invaded lymph nodes than dysplastic lesions but it decreases in HPV-positive cancer patients. Further, LC number correlates with MIP-3α expression.
These findings suggest that LC number is a significant and independent prognostic factor for HNSCC. LC infiltration is increased in cancer lesions but decrease with HPV infection.
头颈部鳞状细胞癌（HNSCC）具有高度的生物学异质性且预后相对较差。烟草和酒精消费与大多数此类癌症的病因有关，但在过去几年中，人乳头瘤病毒（HPV）感染在口咽癌中尤其增加，并成为另一个风险因素。在此，我们评估了HNSCC中朗格汉斯细胞（LCs）的数量，并报告了其与其他风险因素相比的预后能力。
我们的临床系列包括25个无肿瘤的肿瘤周围上皮、64个低级别发育异常、54个高级别发育异常和125个癌样本。通过E6/E7定量聚合酶链反应（qPCR）和p16免疫组织化学检测HPV。在肿瘤内和基质区室以及淋巴结中计数CD1a阳性的LCs。使用免疫组织化学评估癌组织中的巨噬细胞炎性蛋白-3α（MIP-3α）。
单因素Cox回归分析表明，高LC数量与肿瘤内和基质区室中更长的无复发生存期以及基质区室中更长的总生存期相关。就复发而言，烟草和酒精习惯而非HPV状态也与预后不良相关。多因素分析报告基质LC数量是独立于烟草、酒精和HPV状态的强大预后因素。此外，肿瘤和侵袭淋巴结中的LC数量高于发育异常病变，但在HPV阳性癌症患者中会减少。此外，LC数量与MIP-3α表达相关。
这些发现表明LC数量是HNSCC的一个重要且独立的预后因素。LC浸润在癌病变中增加，但随着HPV感染而减少。
