Zhong Jianan, Luo Lijuan, Chen Baowei, Sha Sha, Qing Qing, Tam Nora F Y, Zhang Yong, Luan Tiangang
MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Mar Pollut Bull. 2017 Jan 30;114(2):926-933. doi: 10.1016/j.marpolbul.2016.11.020. Epub 2016 Nov 16.
Alkylated polycyclic aromatic hydrocarbons (PAHs) are abundant in petroleum, and alkylated phenanthrenes are considered as the primary PAHs during some oil spill events. Bacterial strain of Sphingobium sp. MP9-4, isolated from petroleum-contaminated soil, was efficient to degrade 1-methylphenanthrene (1-MP). A detailed metabolism map of 1-MP in this strain was delineated based on analysis of metabolites with gas chromatograph-mass spectrometer (GC-MS). 1-MP was initially oxidized via two different biochemical strategies, including benzene ring and methyl-group attacks. Benzene ring attack was initiated with dioxygenation of the non-methylated aromatic ring via similar degradation pathways of phenanthrene (PHE) by bacteria. For methyl-group attack, mono oxygenase system was involved and more diverse enzymes were needed than that of PHE degradation. This study enhances the understanding of the metabolic pathways of alkylated PAHs and shows the significant potential of Sphingobium sp. MP9-4 for the bioremediation of alkylated PAHs contaminated environments.
烷基化多环芳烃(PAHs)在石油中大量存在,在某些石油泄漏事件中,烷基化菲被视为主要的PAHs。从石油污染土壤中分离出的鞘氨醇单胞菌属细菌菌株MP9-4能够高效降解1-甲基菲(1-MP)。基于气相色谱-质谱联用仪(GC-MS)对代谢产物的分析,描绘了该菌株中1-MP的详细代谢图谱。1-MP最初通过两种不同的生化策略被氧化,包括苯环攻击和甲基攻击。苯环攻击是通过细菌对菲(PHE)的类似降解途径,对未甲基化的芳香环进行双加氧作用而启动的。对于甲基攻击,涉及单加氧酶系统,并且比PHE降解需要更多种类的酶。本研究增进了对烷基化PAHs代谢途径的理解,并显示了鞘氨醇单胞菌属细菌菌株MP9-4在生物修复烷基化PAHs污染环境方面的巨大潜力。