Wu Manli, Li Wei, Dick Warren A, Ye Xiqiong, Chen Kaili, Kost David, Chen Liming
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an, Shaanxi Province 710055, China.
School of Environment and Natural Resources, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, OH 44691, USA.
Chemosphere. 2017 Feb;169:124-130. doi: 10.1016/j.chemosphere.2016.11.059. Epub 2016 Nov 18.
Bioremediation of hydrocarbon degradation in petroleum-polluted soil is carried out by various microorganisms. However, little information is available for the relationships between hydrocarbon degradation rates in petroleum-contaminated soil and microbial population and activity in laboratory assay. In a microcosm study, degradation rate and efficiency of total petroleum hydrocarbons (TPH), alkanes, and polycyclic aromatic hydrocarbons (PAH) in a petroleum-contaminated soil were determined using an infrared photometer oil content analyzer and a gas chromatography mass spectrometry (GC-MS). Also, the populations of TPH, alkane, and PAH degraders were enumerated by a modified most probable number (MPN) procedure, and the hydrocarbon degrading activities of these degraders were determined by the Biolog (MT2) MicroPlates assay. Results showed linear correlations between the TPH and alkane degradation rates and the population and activity increases of TPH and alkane degraders, but no correlation was observed between the PAH degradation rates and the PAH population and activity increases. Petroleum hydrocarbon degrading microbial population measured by MPN was significantly correlated with metabolic activity in the Biolog assay. The results suggest that the MPN procedure and the Biolog assay are efficient methods for assessing the rates of TPH and alkane, but not PAH, bioremediation in oil-contaminated soil in laboratory.
多种微生物可对石油污染土壤中的烃类进行生物修复。然而，在实验室分析中，关于石油污染土壤中烃类降解速率与微生物种群及活性之间的关系，可供参考的信息较少。在一项微观研究中，使用红外光度计油含量分析仪和气相色谱 - 质谱联用仪（GC - MS）测定了石油污染土壤中总石油烃（TPH）、烷烃和多环芳烃（PAH）的降解速率和效率。此外，通过改良的最大可能数（MPN）法对TPH、烷烃和PAH降解菌的数量进行了计数，并通过Biolog（MT2）微孔板分析法测定了这些降解菌的烃类降解活性。结果表明，TPH和烷烃的降解速率与TPH和烷烃降解菌的数量及活性增加之间存在线性关系，但未观察到PAH降解速率与PAH数量及活性增加之间的相关性。通过MPN法测定的石油烃降解微生物数量与Biolog分析中的代谢活性显著相关。结果表明，MPN法和Biolog分析法是评估实验室中石油污染土壤中TPH和烷烃（而非PAH）生物修复速率的有效方法。
