Albuquerque Pedro Paulo Feitosa de, Santos André de Souza, Souza Neto Orestes Luiz de, Kim Pomy de Cássia Peixoto, Cavalcanti Erika Fernanda Torres Samico Fernandes, Oliveira Júnior Mário Baltazar de, Mota Rinaldo Aparecido, Júnior José Wilton Pinheiro
Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Universidade Rural Federal de Pernambuco, Departamento de Medicina Veterinária, Laboratório de Bacterioses, Recife, PE, Brazil.
Braz J Microbiol. 2017 Jan-Mar;48(1):113-117. doi: 10.1016/j.bjm.2016.10.010. Epub 2016 Nov 8.
The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was found in bovine milk samples from the State of Pernambuco. To the best of our knowledge, this is the first report of MAP DNA found in bovine milk in Northeast Brazil. We also demonstrated the qPCR technique is more sensitive than conventional PCR with respect to detection of MAP in milk samples.
本研究旨在利用实时聚合酶链反应（qPCR）和常规PCR检测牛奶样本中副结核分枝杆菌（MAP）的IS900区域，并研究这两种检测方法之间的一致性。从巴西伯南布哥州被认为MAP呈阳性的牛群中总共采集了121份牛奶样本。使用常规PCR在20份样本（16.5%）中检测到MAP DNA，使用qPCR在34份样本（28.1%）中检测到MAP DNA。在所研究的全部6个养殖场中均检测到了MAP DNA。qPCR和常规PCR结果之间存在中度一致性，其中常规PCR相对于qPCR的灵敏度和特异性分别为50%和96.6%。因此，在伯南布哥州的牛奶样本中发现了MAP的IS900区域。据我们所知，这是巴西东北部牛奶中发现MAP DNA的首次报道。我们还证明，在检测牛奶样本中的MAP方面，qPCR技术比常规PCR更灵敏。
