Sequeira Marilyn P, Sinha Sapna, Motiwalla Mustafa J, Rao Venkatramanan G, D'Souza Jacinta S
UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
UM-DAE Center for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai, India.
Biochem Biophys Res Commun. 2017 Jan 22;482(4):610-614. doi: 10.1016/j.bbrc.2016.11.081. Epub 2016 Nov 16.
The long-flagella mutants (lf1, lf2, lf3 and lf4) of Chlamydomonas reinhardtii are defective in proteins that are required for the assembly of normal flagella, their phenotype being long flagella. In a previous study, we biophysically characterized these mutants for their waveform patterns, swimming speeds, beat frequencies and correlated these parameters with their flagellar lengths. We found an anomaly in this correlation and set out to explore the underlying molecular significance, if any. The diverse inner dynein isoforms are the flagellar motors that convert the chemical energy of ATP into the mechanical energy of motility; we probed the presence of one of these isoforms (DHC11, which might help in bend initiation) in the lf mutants and compared it with the wild-type. Our studies show that the ratio of DHC11 is defective in the long-flagella mutants of Chlamydomonas reinhardtii.
莱茵衣藻的长鞭毛突变体（lf1、lf2、lf3和lf4）在正常鞭毛组装所需的蛋白质方面存在缺陷，其表型为长鞭毛。在之前的一项研究中，我们对这些突变体的波形模式、游动速度、摆动频率进行了生物物理表征，并将这些参数与其鞭毛长度相关联。我们发现这种相关性存在异常，并着手探索其潜在的分子意义（如果有的话）。多种内动力蛋白异构体是将ATP的化学能转化为运动机械能的鞭毛马达；我们检测了这些异构体之一（DHC11，可能有助于弯曲起始）在lf突变体中的存在情况，并将其与野生型进行比较。我们的研究表明，莱茵衣藻长鞭毛突变体中DHC11的比例存在缺陷。
