吗啡通过提高生存素水平增强肾细胞癌的侵袭性。
Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level.
作者信息
Ma Yabing, Ren Zhongzhong, Ma Shuyong, Yan Wenjun, He Man, Wang Dong, Ding Peiyan
机构信息
a Department of Anesthesia , Gansu provincial People's Hospital , Lanzhou , Gansu Province , PR China.
b Departments of Medical Record , Shaanxi Provincial People's Hospital , Xian, Shaanxi Province , China.
出版信息
Ren Fail. 2017 Nov;39(1):258-264. doi: 10.1080/0886022X.2016.1256322. Epub 2016 Nov 21.
BACKGROUND
Morphine is an opioid analgesic drug often used for pain relief in cancer patients. However, there is growing evidence that morphine may modulate tumor growth, progression and metastasis. Unfortunately, the results obtained by these studies are still contradictory.
METHODS
In this study, we investigated the effect of morphine in human clear cell renal cell carcinoma 786-O, RLC-310 cells and whether morphine affects on tumor growth in human clear cell renal cell carcinoma 786-O, RLC-310 cells. The cell proliferation was determined by MTT assay, cell proliferation, migration and invasion assays. Immunofluorescence staining and Q-PCR was used to determine the Survivin expression.
RESULTS
It was shown that morphine enhances proliferation of 786-O, RLC-310 cells, whereas morphine promoted the growth and aggressive phenotype of 786-O and RLC-310 cells in vitro though Survivin-dependent signaling.
CONCLUSIONS
Our data showed that morphine promotes RCC growth and increases RCC progression via over-expression of Survivin.
背景
吗啡是一种阿片类镇痛药，常用于缓解癌症患者的疼痛。然而，越来越多的证据表明吗啡可能会调节肿瘤的生长、进展和转移。不幸的是，这些研究获得的结果仍然相互矛盾。
方法
在本研究中，我们研究了吗啡对人透明细胞肾细胞癌786-O、RLC-310细胞的影响，以及吗啡是否影响人透明细胞肾细胞癌786-O、RLC-310细胞的肿瘤生长。通过MTT法、细胞增殖、迁移和侵袭试验来测定细胞增殖。采用免疫荧光染色和Q-PCR法测定Survivin的表达。
结果
结果表明，吗啡可增强786-O、RLC-310细胞的增殖，而吗啡通过依赖Survivin的信号通路在体外促进786-O和RLC-310细胞的生长和侵袭性表型。
结论
我们的数据表明，吗啡通过Survivin的过表达促进肾细胞癌的生长并增加肾细胞癌的进展。
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