来自狩猎巨蟹蛛的富含肽的毒液能有效抑制昆虫的电压门控钠通道。

Peptide-rich venom from the spider Heteropoda venatoria potently inhibits insect voltage-gated sodium channels.

作者信息

Huang Yazhou, Wu Xinzhou, Zhang Peng, Duan Zhigui, Zhou Xi, Chen Minzhi, Farooq Athar, Liang Songping, Liu Zhonghua

机构信息

The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.

The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China; The Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.

出版信息

Toxicon. 2017 Jan;125:44-49. doi: 10.1016/j.toxicon.2016.11.252. Epub 2016 Nov 17.

DOI:10.1016/j.toxicon.2016.11.252

PMID:27867092

Abstract

Heteropoda venatoria is a venomous spider species distributed worldwide and has a characteristic habit of feeding on insects. Reverse-phase high-performance liquid chromatography and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analyses revealed that H. venatoria venom contains hundreds of peptides with a predominant molecular weights of 3000-5000 Da. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD was 28.18 μg/g of body weight in the cockroach. It was found that the venom had potent inhibitory effect on voltage-gated sodium channels (VGSCs) in Periplaneta americana cockroach dorsal unpaired median (DUM) neurons with an IC values of 6.25 ± 0.02 μg/mL. However, 100 μg/mL venom only partially blocked VGSC currents in rat dorsal root ganglion cells, a much lower inhibitory effect than that on DUM VGSCs. Our results indicate that the venom of H. venatoria contains diverse neurotoxins that might become new leads for bioinsecticides.

摘要

斑络新妇是一种分布于全球的有毒蜘蛛物种，具有以昆虫为食的独特习性。反相高效液相色谱和基质辅助激光解吸/电离飞行时间质谱分析表明，斑络新妇毒液含有数百种肽，主要分子量为3000 - 5000 Da。腹腔注射毒液对蟑螂有严重毒性作用，在较高浓度下可导致死亡。蟑螂的半数致死量为28.18 μg/g体重。研究发现，该毒液对美洲大蠊背中无对神经元的电压门控钠通道（VGSCs）有强大的抑制作用，半数抑制浓度值为6.25±0.02 μg/mL。然而，100 μg/mL的毒液仅部分阻断大鼠背根神经节细胞中的VGSC电流，其抑制作用远低于对背中无对神经元VGSCs的抑制作用。我们的结果表明，斑络新妇毒液含有多种神经毒素，可能成为生物杀虫剂的新线索。