Song Hai-bing, Wang Bin, Chen Ren-jie, Zheng Xiao-ya, Yu Shi-bo, Lan Tao
1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Yi Chuan. 2016 Nov 20;38(11):1012-1019. doi: 10.16288/j.yczz.16-114.
We obtained a glabrous leaf and hull mutant from a population of radiation mutagenesis of an indica rice cultivar R401. The mutant produced smooth leaves and hairless glumes under normal growth conditions. An F population was developed from a cross between a japonica cultivar Nipponbare and the glabrous leaf and hull mutant. By investigating the performance of the F population, we found that the mutant phenotype was controlled by a single recessive gene, temporarily designated GLR3. Bulked segregant analysis (BSA) based on the F mapping population revealed that GLR3 is located on chromosome 6. By analyzing 417 typical glabrous leaf F plants using molecular markers, GLR3 was mapped to a 0.2 cM interval between InDel markers ID27101 and ID27199, and the physical distance between the two markers is 98 kb. Thus we have mapped the gene GLR3, and our work will provide basis for future mechanistic analysis of GLR3 function.
我们从籼稻品种R401的辐射诱变群体中获得了一个叶片和颖壳无毛突变体。该突变体在正常生长条件下叶片光滑且颖壳无毛。通过粳稻品种日本晴与叶片和颖壳无毛突变体杂交构建了一个F群体。通过对F群体的表型调查，我们发现该突变体表型受一对隐性基因控制，暂时命名为GLR3。基于F定位群体的混合分离分析法（BSA）表明GLR3位于第6号染色体上。利用分子标记对417株典型的叶片无毛F植株进行分析，将GLR3定位到InDel标记ID27101和ID27199之间0.2 cM的区间内，两个标记间的物理距离为98 kb。至此我们完成了GLR3基因的定位，这将为今后GLR3功能的机制分析提供依据。
