Genetic analysis and high-resolution mapping of a premature senescence gene Pse(t) in rice (Oryza sativa L.).

A rice mutant, designated pse(t) (premature senescence, tentatively), was isolated from a T-DNA-inserted transgenic population. Senescence advanced more markedly in pse(t) than in wild-type ('Zhonghua 11', japonica) plants. Genetic analysis of pse(t) revealed that the premature senescence mutation was controlled by a single recessive nuclear gene, but that it was not induced by T-DNA insertion. In an effort to understand the genetic and molecular basis underlying premature senescence in rice, a map-based cloning strategy was used to localize Pse(t). High-resolution mapping of the Pse(t) locus was carried out using simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) markers. An F2 population, comprising 1691 pse(t) individuals derived from a cross of the pse(t) mutant with 'Longtepu' (indica), was constructed. Several new polymorphism markers were developed in this study. Genetic linkage analysis showed that the Pse(t) gene was located on the long arm of chromosome 7. It was found that the Pse(t) gene cosegregated with 3 markers and was flanked by markers SS22 and PP21. Thus, the Pse(t) gene is located within a genetic distance of 0.15 cM, corresponding to a physical distance of 220 kb. These findings provide the basic information that can be used for the final isolation of this gene in the rice premature-senescence pathway.