OsACOS12是拟南芥酰基辅酶A合成酶5的直系同源基因，在水稻花粉外壁形成和花药发育中起重要作用。
OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice.
作者信息
Li Yueling, Li Dandan, Guo Zongli, Shi Qiangsheng, Xiong Shuangxi, Zhang Cheng, Zhu Jun, Yang Zhongnan
机构信息
College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China.
出版信息
BMC Plant Biol. 2016 Nov 21;16(1):256. doi: 10.1186/s12870-016-0943-9.
BACKGROUND
Sporopollenin is a major component of the pollen exine pattern. In Arabidopsis, acyl-CoA synthetase5 (ACOS5) is involved in sporopollenin precursor biosynthesis. In this study, we identified its orthologue, OsACOS12, in rice (Oryza sativa) and compared the functional conservation of ACOS in rice to Arabidopsis.
RESULTS
Sequence analysis showed that OsACOS12 shares 63.9 % amino acid sequence identity with ACOS5. The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice. In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level. The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules. OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis.
CONCLUSIONS
OsACOS12 is an orthologue of ACOS5 that is essential for sporopollenin synthesis in rice. ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species.
背景
孢粉素是花粉外壁模式的主要成分。在拟南芥中，酰基辅酶A合成酶5（ACOS5）参与孢粉素前体的生物合成。在本研究中，我们在水稻（Oryza sativa）中鉴定出其直系同源物OsACOS12，并比较了水稻和拟南芥中ACOS的功能保守性。
结果
序列分析表明，OsACOS12与ACOS5的氨基酸序列同一性为63.9%。由LOC_Os04g24530中的提前终止密码子引起的osacos12突变导致性外壁缺陷，从而在水稻中产生雄性不育表型。原位杂交显示，OsACOS12在绒毡层细胞和小孢子中转录水平表达。OsACOS12-GFP的定位表明，OsACOS12蛋白在绒毡层细胞和花药室中积累。由ACOS5启动子驱动的OsACOS12可以部分恢复拟南芥中acos5突变体的雄性育性。
结论
OsACOS12是ACOS5的直系同源物，对水稻中孢粉素的合成至关重要。ACOS5和OsACOS12在单子叶和双子叶植物物种的花粉壁形成中是保守的。
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