一种工程化转录激活样效应蛋白对甲基化DNA的序列特异性识别。
Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein.
作者信息
Tsuji Shogo, Futaki Shiroh, Imanishi Miki
机构信息
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
出版信息
Chem Commun (Camb). 2016 Dec 6;52(99):14238-14241. doi: 10.1039/c6cc06824c.
PMID:27872906
Abstract
A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.
摘要
通过筛选一个在重复可变双残基及其相邻残基处含有随机氨基酸的TALE重复文库，创建了一个5mC选择性TALE重复序列。新的重复序列显示出高5mC识别能力。含有新重复序列的人工TALE以基因组甲基化状态依赖的方式激活了一个内源基因。
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