Bonn Florian, Maass Sandra, Becher Dörte
Department for Microbial Proteomics, Institute for Microbiology, Ernst-Moritz-Arndt University of Greifswald, Friedrich-Ludwig-Jahn-Straße 15, Greifswald, 17489, Germany.
Methods Mol Biol. 2017;1520:281-289. doi: 10.1007/978-1-4939-6634-9_17.
Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this chapter, a universal protocol for gel free absolute protein quantification in bacterial systems is described, which can be used for sample preparation prior to miscellaneous mass-spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research.
绝对蛋白质定量是系统生物学方法以及阐明多蛋白复合物化学计量学的重要工具。在本章中，将描述一种用于细菌系统中无凝胶绝对蛋白质定量的通用方案，该方案可用于诸如AQUA、Hi3和emPAI等基于质谱的各种定量工作流程之前的样品制备。此外，还重点关注了抗生素应激研究中的特定挑战。
