Heuts Frank, Nagy Noemi
Institute of Immunity and Transplantation, University College London Medical School, London, UK.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Nobels väg 16, Stockholm, SE-171 77, Sweden.
Methods Mol Biol. 2017;1532:229-240. doi: 10.1007/978-1-4939-6655-4_17.
Recent developments in mouse models that harbor part of a human immune system have proved extremely valuable to study the in vivo immune response to human specific pathogens such as Epstein-Barr virus. Over the last decades, advances in immunodeficient mouse strains that can be used as recipients for human immune cells have greatly enhanced the use of these models. Here, we describe the generation of mice with reconstituted human immune system (HIS mice) using immunocompromised mice transplanted with human CD34 hematopoietic stem cells. We will also describe how such mice, in which human immune cells are generated de novo, can be used to study EBV infection.
携带部分人类免疫系统的小鼠模型的最新进展已被证明对研究针对人类特异性病原体（如爱泼斯坦-巴尔病毒）的体内免疫反应极具价值。在过去几十年中，可用作人类免疫细胞受体的免疫缺陷小鼠品系的进展极大地促进了这些模型的应用。在这里，我们描述了使用移植了人类CD34造血干细胞的免疫受损小鼠生成具有重建人类免疫系统的小鼠（人类免疫系统重建小鼠）的方法。我们还将描述如何利用这些从头产生人类免疫细胞的小鼠来研究EBV感染。
