Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Japan.
Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Sagamihara, Japan.
Allergy. 2017 Jun;72(6):918-926. doi: 10.1111/all.13091. Epub 2017 Jan 24.
Booklice, belonging to the order Psocoptera, are small household insect pests that are distributed worldwide. Liposcelis bostrychophila, a common home-inhabiting species of booklouse, infests old books, sheets of paper, and stored food. Recent entomological and serological studies demonstrated that L. bostrychophila accounted for the majority of detectable insects in house dust and could be a potent inducer of respiratory allergy. Our recent proteomic analysis identified a potent allergenic protein from L. bostrychophila, designated Lip b 1, and determined its partial amino acid sequences.
Cloning of cDNAs for Lip b 1 was performed by large-scale transcriptome analysis (RNA-seq) and subsequent reverse transcription polymerase chain reaction. The full-length amino acid sequences deduced from Lip b 1 cDNAs were bioinformatically analyzed. The recombinant proteins of glutathione S-transferase (GST)-fused Lip b 1 were analyzed by Western blot and enzyme-linked immunosorbent assay.
Lip b 1 cDNAs encoding two types of 254-amino acid proteins were cloned. The clones shared 87% identity, and the deduced molecular weights and isoelectric points were consistent with those determined in our previous study. The two types of Lip b 1 proteins in the GST-fused form were similarly reactive with sera from allergic patients sensitized with L. bostrychophila.
Lip b 1 is a novel protein possibly causing booklouse allergy.
书虱属于啮虫目，是分布于全世界的小型家居昆虫害虫。嗜卷书虱是常见的家居书虱，它会侵害旧书、纸张和储存的食物。最近的昆虫学和血清学研究表明，嗜卷书虱在房屋灰尘中占大多数可检测到的昆虫，并可能是呼吸道过敏的强烈诱因。我们最近的蛋白质组学分析从嗜卷书虱中鉴定出一种有效的过敏原蛋白，命名为 Lip b 1，并确定了其部分氨基酸序列。
通过大规模转录组分析（RNA-seq）和随后的反转录聚合酶链反应来克隆 Lip b 1 的 cDNA。从 Lip b 1 cDNA 推导的全长氨基酸序列进行生物信息学分析。谷胱甘肽 S-转移酶（GST）融合的 Lip b 1 重组蛋白通过 Western blot 和酶联免疫吸附试验进行分析。
克隆了编码两种 254 个氨基酸蛋白的 Lip b 1 cDNA。这些克隆体具有 87%的同一性，推导的分子量和等电点与我们之前的研究一致。以 GST 融合形式存在的两种 Lip b 1 蛋白与对嗜卷书虱过敏的患者的血清反应相似。
Lip b 1 是一种可能引起书虱过敏的新型蛋白。
