多聚鸟氨酸通过促进α-辅肌动蛋白 4 与肌动蛋白丝结合增强神经干细胞/祖细胞的迁移。

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

机构信息

Department of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.

出版信息

Sci Rep. 2016 Nov 22;6:37681. doi: 10.1038/srep37681.

The recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN). Here, we demonstrate that PO could promote migration of NSPCs in vitro, and the underlying mechanism is PO activates α-Actinins 4 (ACTN4), which is firstly certified to be expessed in NSPCs, to promote filopodia formation and therefore enhances NSPCs migration. Taken together, PO might serve as a better candidate for transplanted biomaterials in the regenerative therapeutic strategy, compared with PLL and FN.

神经干细胞/祖细胞（NSPCs）的募集用于损伤后的大脑修复是一种很有前途的再生治疗策略。该策略涉及增强 NSPCs 的增殖、迁移和神经元分化。迄今为止，缺乏促进这些过程以增强神经再生的生物材料是细胞替代疗法的一个障碍。我们之前的研究表明，与多聚-L-赖氨酸（PLL）和纤维连接蛋白（FN）相比，在聚-L-鸟氨酸（PO）上生长的 NSPCs 增殖更旺盛，分化为更多的神经元。在这里，我们证明 PO 可以促进 NSPCs 的体外迁移，其潜在机制是 PO 激活首先在 NSPCs 中表达的α-辅肌动蛋白 4（ACTN4），以促进丝状伪足的形成，从而增强 NSPCs 的迁移。总之，与 PLL 和 FN 相比，PO 可能成为再生治疗策略中移植生物材料的更好候选物。