Fernan A, Cayzer C J, Cooksley W G
Liver Unit, University of Queensland, Brisbane, Australia.
Clin Exp Immunol. 1989 May;76(2):222-6.
This report describes a study of in vitro proliferative and antibody responses to the hepatitis B virus surface antigen (HBsAg) of lymphocytes from chronic HBsAg carriers, subjects with naturally acquired immunity, and responders to the hepatitis B vaccine. Peripheral blood T and B lymphocytes were cultured with a wide range of concentrations of HBsAg (0.025-250 ng/ml). We were unable to detect HBsAg-specific proliferation or antibody synthesis in any of the subject groups studied, despite the use of a range of antigen concentrations, cell ratios and culture periods. The addition of recombinant interleukin 2 (rIL-2) or T cell growth factor at either initiation or day 3 of culture enhanced proliferative responses, but in an antigen-independent manner. In contrast to the proliferation observed following the addition of IL-2, the absence of responses to specific antigen suggest there may be low numbers of HBsAg-specific precursors in the peripheral blood.
本报告描述了一项针对慢性乙肝表面抗原（HBsAg）携带者、自然获得免疫力者以及乙肝疫苗应答者的淋巴细胞对乙肝病毒表面抗原（HBsAg）的体外增殖和抗体反应的研究。外周血T淋巴细胞和B淋巴细胞用一系列浓度（0.025 - 250 ng/ml）的HBsAg进行培养。尽管使用了一系列抗原浓度、细胞比例和培养时间，但在所研究的任何受试者组中，我们均未能检测到HBsAg特异性增殖或抗体合成。在培养开始时或培养第3天添加重组白细胞介素2（rIL - 2）或T细胞生长因子可增强增殖反应，但这种增强是以抗原非依赖的方式进行的。与添加IL - 2后观察到的增殖情况相反，对特异性抗原无反应表明外周血中HBsAg特异性前体细胞数量可能较少。
