Chang H L, Zaroukian M H, Morrison M H, Esselman W J
Department of Microbiology, Michigan State University, East Lansing.
Nat Immun Cell Growth Regul. 1989;8(2):89-99.
Plastic-adherent lymphokine-activated natural killer (LANK) cells were generated from nylon wool-nonadherent murine splenocytes cultured in recombinant interleukin-2 (IL-2). Under such conditions, adherent lymphokine-activated killer cells capable of killing natural killer (NK)-resistant targets were not generated. Adherent LANK cells proliferated rapidly and closely resembled NK cells in their morphology, cytotoxic reactivity, and surface marker expression. Mice with severe combined immunodeficiency (scid) were used to generate adherent LANK cells to define the role of T cells in LANK cell development. Scid lymphocytes responded to IL-2 by becoming adherent LANK cells with potent NK-like activity, suggesting that soluble lymphokines other than IL-2 that may have been produced by T cells were not required for the generation of LANK cell activity in mice.
塑料贴壁的淋巴因子激活的自然杀伤（LANK）细胞是由在重组白细胞介素-2（IL-2）中培养的尼龙毛非贴壁小鼠脾细胞产生的。在这种条件下，不能产生能够杀伤自然杀伤（NK）抗性靶标的贴壁淋巴因子激活的杀伤细胞。贴壁的LANK细胞迅速增殖，在形态、细胞毒性反应性和表面标志物表达方面与NK细胞非常相似。利用严重联合免疫缺陷（scid）小鼠产生贴壁的LANK细胞，以确定T细胞在LANK细胞发育中的作用。Scid淋巴细胞通过变成具有强大NK样活性的贴壁LANK细胞来对IL-2作出反应，这表明在小鼠中产生LANK细胞活性不需要T细胞可能产生的除IL-2之外的可溶性淋巴因子。
