Nordström C H, Siesjö B K
Stroke. 1978 Jul-Aug;9(4):327-35. doi: 10.1161/01.str.9.4.327.
Changes in cerebral cortex concentrations of high-energy phosphates, glycolytic metabolites, citric acid cycle intermediates, associated amino acids, and ammonia, were studied after 5, 15 and 30 min of incomplete ischemia in rats anesthetized with 70% N2O or 150 mg.kg-1 of phenobartibal. Previous results have shown that with this type of ischemia (bilateral carotid artery occlusion combined with reduction in blood pressure to 50 mm Hg) cortical blood flow is reduced to below 10% of nitrous oxide values, whether animals are anesthetized with 70% N2O or 150 mg.kg-1 of phenobarbital. In animals under 70% N2O, changes in tissue concentrations of phosphocreatine, ATP, ADP and AMP were similar to those previously obtained in complete ischemia. However, some glucose remained in the tissue, and the lactate concentrations gradually rose to reach excessive values. Changes occuring in glycolytic and citric acid cycle intermediates were similar to those seen in complete ischemia but, after 30 min, there was some reduction in the pool size of amino acids. In those animals given phenobarbital and which lost all EEG activity during ischemia, changes in cerebral metabolites were virtually identical to those observed in nitrous oxide-anesthetized animals. However, some animals exposed to 5 or 15 min of ischemia had some remaining EEG activity. In these, cerebral energy state was significantly less deranged, and levels of glycogen, glucose and pyruvate were higher.
在用70%一氧化二氮或150mg/kg苯巴比妥麻醉的大鼠中，研究了不完全缺血5、15和30分钟后脑皮质中高能磷酸盐、糖酵解代谢产物、柠檬酸循环中间体、相关氨基酸和氨的浓度变化。先前的结果表明，对于这种类型的缺血（双侧颈动脉闭塞并将血压降至50mmHg），无论动物是用70%一氧化二氮还是150mg/kg苯巴比妥麻醉，皮质血流量都会降至低于一氧化二氮值的10%。在70%一氧化二氮麻醉的动物中，磷酸肌酸、ATP、ADP和AMP的组织浓度变化与先前在完全缺血中获得的变化相似。然而，组织中仍残留一些葡萄糖，乳酸浓度逐渐升高至过高值。糖酵解和柠檬酸循环中间体的变化与完全缺血中所见的相似，但在30分钟后，氨基酸池大小有所减少。在给予苯巴比妥且在缺血期间失去所有脑电图活动的动物中，脑代谢产物的变化与在一氧化二氮麻醉的动物中观察到的几乎相同。然而，一些经历5或15分钟缺血的动物仍有一些残留的脑电图活动。在这些动物中，脑能量状态的紊乱程度明显较轻，糖原、葡萄糖和丙酮酸水平较高。
