Liu Bowen, Teng Zihao, Wang Jianfeng, Lu Gejin, Deng Xuming, Li Li
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
Fitoterapia. 2017 Jan;116:45-50. doi: 10.1016/j.fitote.2016.11.012. Epub 2016 Nov 22.
The foodborne pathogenic bacterial species Listeria monocytogenes (L. monocytogenes) has caused incalculable damages to public health, and its successful infection requires various virulence factors, including Listeriolysin O (LLO). By forming pores in phagosomal membranes and even in some organelles, LLO plays an indispensable role in the ability of L. monocytogenes to escape from host immune attacks. Because of its critical role, LLO offers an appropriate therapeutic target against L. monocytogenes infection. Here, lutein, a natural small molecule existing widely in fruits and vegetables, is demonstrated as an effective inhibitor of LLO that works by blocking its oligomerization during invasion without showing significant bacteriostatic activity. Further assays applying lutein in cell culture models of invasion and in animal models showed that lutein could effectively inhibit L. monocytogenes infection. Overall, our results indicate that lutein may represent a promising and novel therapeutic agent against L. monocytogenes infection.
食源性病原体单核细胞增生李斯特菌（L. monocytogenes）已对公众健康造成了难以估量的损害，其成功感染需要多种毒力因子，包括李斯特菌溶血素O（LLO）。通过在吞噬体膜甚至某些细胞器中形成孔道，LLO在单核细胞增生李斯特菌逃避宿主免疫攻击的能力中发挥着不可或缺的作用。由于其关键作用，LLO为对抗单核细胞增生李斯特菌感染提供了一个合适的治疗靶点。在此，叶黄素，一种广泛存在于水果和蔬菜中的天然小分子，被证明是LLO的有效抑制剂，其作用机制是在入侵过程中阻断LLO的寡聚化，且不表现出显著的抑菌活性。进一步在入侵细胞培养模型和动物模型中应用叶黄素的实验表明，叶黄素可以有效抑制单核细胞增生李斯特菌感染。总体而言，我们的结果表明，叶黄素可能是一种有前景的新型抗单核细胞增生李斯特菌感染治疗剂。
