冷冻保存条件下肠出血性大肠杆菌O157的活的非可培养状态及毒素基因表达
Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation.
作者信息
Liu Junyan, Zhou Rong, Li Lin, Peters Brian M, Li Bing, Lin Chii-Wann, Chuang Tsung-Liang, Chen Dingqiang, Zhao Xihong, Xiong Zhiyong, Xu Zhenbo, Shirtliff Mark E
机构信息
College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.
College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
出版信息
Res Microbiol. 2017 Apr;168(3):188-193. doi: 10.1016/j.resmic.2016.11.002. Epub 2016 Nov 21.
As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at -20 °C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety.
作为全球主要的食源性病原体，大肠杆菌能够产生毒素，直接导致严重的人类疾病。然而，常规方法无法检测食品和原材料中存活但不可培养（VBNC）的细菌，从而导致假阴性鉴定。在本研究中，大肠杆菌O157菌株在-20°C冷冻保存后获得了VBNC状态，有无冻融处理；观察到形态为较短的杆状，毒素表达仍处于相对较高水平。还验证了用于VBNC检测的PMA-PCR检测方法。因此，这些结果表明，VBNC大肠杆菌O157菌株可能对公共卫生和食品安全构成重大威胁。
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