De Nicola A F, von Lawzewitsch I, Kaplan S E, Libertun C
J Natl Cancer Inst. 1978 Sep;61(3):753-63.
The hormone function, the metabolism of nucleic acids, and the ultrastructure of estrogen-induced pituitary tumors and of normal glands were examined in male F344 rats. The tumors had a high capacity for prolactin (PRL) synthesis, and the plasma levels of PRL were elevated 65-fold to 100-fold in the tumor-bearing animals. Uridine uptake and phosphorylation to nucleotides, as well as uridine incorporation into total RNA, were similar in tumors and normal glands, whereas [3H]thymidine incorporation into DNA was double in the former group as compared to the latter. After a [3H]uridine pulse, labeled RNA turnover was different in tumors and normal glands. Electron microscopy of the tumors revealed hypertrophy, degranulation, and hyperplasia of cells producing PRL with proliferation of their ergastoplasm in whorls. Other pituitary cell types were reduced in number. It is suggested that the whorl configuration caused the high rate of protein and PRL synthesis as well as the changes in RNA metabolism displayed by the tumors.
对雄性F344大鼠雌激素诱导的垂体肿瘤及正常腺体的激素功能、核酸代谢和超微结构进行了研究。这些肿瘤具有高催乳素（PRL）合成能力，荷瘤动物血浆PRL水平升高了65倍至100倍。肿瘤和正常腺体中尿苷摄取及磷酸化生成核苷酸，以及尿苷掺入总RNA的情况相似，而与后者相比，前者中[3H]胸腺嘧啶核苷掺入DNA的量增加了一倍。经[3H]尿苷脉冲处理后，肿瘤和正常腺体中标记RNA的周转情况不同。肿瘤的电子显微镜检查显示，分泌PRL的细胞肥大、脱颗粒和增生，其内质网呈螺旋状增殖。其他垂体细胞类型数量减少。提示螺旋状结构导致了肿瘤中蛋白质和PRL的高合成率以及RNA代谢的变化。
