Carrillo Catherine D, Kenwell Robyn, Iugovaz Irene, Oyarzabal Omar A
Canadian Food Inspection Agency, 960 Carling Avenue Bldg # 22, Ottawa, ON, Canada, K1A 0Y9.
Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON, Canada, K1A 0K9.
Methods Mol Biol. 2017;1512:9-18. doi: 10.1007/978-1-4939-6536-6_2.
The recovery of Campylobacter species from food and environmental sources is challenging due to the slow growth of these bacteria and the need to suppress competing organisms during the isolation procedures. The addition of multiple selective antimicrobials to growth media can negatively impact recovery of some Campylobacter spp. Here, we describe our current method for the isolation of thermotolerant Campylobacter species, mainly C. jejuni and C. coli, from food and environmental samples. We emphasize the use of membrane filtration during plating for the specific isolation of Campylobacter spp. and a reduced use of antimicrobial supplements throughout the whole isolation process.
由于弯曲杆菌属细菌生长缓慢,且在分离过程中需要抑制竞争性微生物,从食品和环境来源中分离弯曲杆菌属具有挑战性。在生长培养基中添加多种选择性抗菌剂可能会对某些弯曲杆菌属物种的回收率产生负面影响。在此,我们描述了我们目前从食品和环境样本中分离耐热弯曲杆菌属物种(主要是空肠弯曲菌和结肠弯曲菌)的方法。我们强调在平板接种过程中使用膜过滤来特异性分离弯曲杆菌属物种,并在整个分离过程中减少抗菌补充剂的使用。