Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality, Bioscience Engineering, Ghent University, Belgium.
Int J Food Microbiol. 2009 Nov 15;135(3):248-53. doi: 10.1016/j.ijfoodmicro.2009.09.007. Epub 2009 Sep 16.
A comparative study examining Bolton broth and Preston broth for enrichment and reliable detection of Campylobacter jejuni (both healthy and freeze stressed cells) was performed. Tested as pure cultures, Bolton broth enabled faster resuscitation and growth of C. jejuni compared to Preston broth. When C. jejuni was co-incubated with extended-spectrum-beta-lactamase (ESBL) producing Escherichia coli isolated from Belgian poultry meat preparations, the latter dominated in the Bolton enrichment broth and crowded the mCCDA plates. This resulted in the inability to recover C. jejuni by ISO 10272-1:2006 standard method. Preston broth did not support the growth of the ESBL E. coli isolates, but showed longer detection time of C. jejuni compared to Bolton broth. The use of the same antibiotic (sodium cefoperazone) in Bolton broth and in mCCDA plates may explain the problems encountered for detection of C. jejuni, as high numbers of ESBL E. coli present after enrichment in Bolton broth, also caused overgrowth and masked the few C. jejuni colonies present on the mCCDA plates. The use of Campylobacter spp. specific real-time PCR circumvented these problems and enabled rapid detection of the pathogen after 24h enrichment in both Bolton and Preston broth, for both healthy and freeze stressed cells.
进行了一项比较研究，考察了 Bolton 肉汤和 Preston 肉汤在富集和可靠检测空肠弯曲菌（包括健康和冷冻应激细胞）方面的效果。在纯培养物测试中，与 Preston 肉汤相比，Bolton 肉汤能够更快地复苏和生长空肠弯曲菌。当空肠弯曲菌与从比利时禽肉制品中分离出的产超广谱β-内酰胺酶（ESBL）的大肠杆菌共同孵育时，后者在 Bolton 富集肉汤中占优势，并挤满 mCCDA 平板。这导致无法按照 ISO 10272-1:2006 标准方法回收空肠弯曲菌。Preston 肉汤不支持 ESBL 大肠杆菌分离株的生长，但与 Bolton 肉汤相比，检测空肠弯曲菌的时间更长。在 Bolton 肉汤和 mCCDA 平板中使用相同的抗生素（头孢哌酮钠）可能解释了在检测空肠弯曲菌时遇到的问题，因为在 Bolton 肉汤中富集后存在大量的 ESBL 大肠杆菌，也导致过度生长并掩盖了 mCCDA 平板上存在的少量空肠弯曲菌菌落。使用弯曲菌属特异性实时 PCR 绕过了这些问题，并能够在 Bolton 和 Preston 肉汤中分别对健康和冷冻应激细胞进行 24 小时富集后快速检测病原体。
