Zimber Z, Perk K
J Natl Cancer Inst. 1978 Sep;61(3):793-7.
Of 11 male baby chicks treated twice with 3 mg of 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitroso-trans-urea (once on the day of hatching and once on the day after), 9 showed absolute lack of spermatogenesis and 2 showed severe inhibition when examined at the age of 6 months. Plasma testosterone level was not altered, however, and histologic examination revealed well-organized testicular interstitial tissue with an abundance of Leydig's cells. In the lining of the seminiferous tubules only Sertoli's cells were present. The weight of the testes from treated animals was only about 10% of control weight. These findings emphasized the possibility that the nitrosoureas cause severe damage to the reproductive system in man.
在11只雄性雏鸡中，用3毫克1-(2-氯乙基)-3-(4-甲基环己基)-1-亚硝基反式脲处理两次（一次在孵化当天，一次在第二天），6个月大时检查发现，9只完全缺乏精子发生，2只表现出严重抑制。然而，血浆睾酮水平未改变，组织学检查显示睾丸间质组织结构良好，有大量的莱迪希细胞。生精小管内衬仅存在支持细胞。处理过的动物的睾丸重量仅约为对照重量的10%。这些发现强调了亚硝基脲类药物可能对人类生殖系统造成严重损害的可能性。
