Lubet R A, Carlson D E
J Natl Cancer Inst. 1978 Sep;61(3):897-903.
The murine plasmacytoma MOPC 104E was susceptible to cytotoxic therapy in female inbred BALB/c mice. Palpable subcutaneous tumors (0.6--1.2 X 10(8) cells) could be cured with a single administration of cyclophosphamide (5--250 mg/kg) or localized irradiation (800--2,400 R). Clonogenic assay showed that, following minimal curative doses of cyclophosphamide or radiation, 0.5--1.5 X 10(6) tumor cells should remain viable. Control animals succumbed to progressive, invariably lethal tumor growth after they were given sc injections of 2--3 X 10(3) tumor cells. Minimal doses of cyclophosphamide, which were curative in control tumor-bearing animals, were ineffective in treating tumor-bearing animals immunosuppressed by 450 R whole-body irradiation. Subsequent experiments measured the ability of animals cured of the murine plasmacytoma to reject secondary challenge with the same tumor. These experiments demonstrated and partially quantitated the substantial role of the immune response in effecting tumor cure following radiotherapy or chemotherapy.
小鼠浆细胞瘤MOPC 104E对雌性近交系BALB/c小鼠的细胞毒性疗法敏感。可触及的皮下肿瘤（0.6 - 1.2×10⁸个细胞）通过单次给予环磷酰胺（5 - 250毫克/千克）或局部照射（800 - 2400伦琴）即可治愈。克隆形成试验表明，在给予最低治愈剂量的环磷酰胺或辐射后，仍应有0.5 - 1.5×10⁶个肿瘤细胞存活。对照组动物在皮下注射2 - 3×10³个肿瘤细胞后，会因肿瘤进行性生长而最终死亡。在对照组荷瘤动物中具有治愈效果的最低剂量环磷酰胺，对经450伦琴全身照射免疫抑制的荷瘤动物无效。后续实验检测了治愈小鼠浆细胞瘤的动物排斥相同肿瘤二次攻击的能力。这些实验证明并部分量化了免疫反应在放疗或化疗后实现肿瘤治愈中的重要作用。
