重组和转基因小鼠中杂交Ia二聚体（EαAβ）的体内表达及功能
In vivo expression and function of hybrid Ia dimers (E alpha A beta) in recombinant and transgenic mice.
作者信息
Anderson G D, David C S
机构信息
Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905.
出版信息
J Exp Med. 1989 Sep 1;170(3):1003-8. doi: 10.1084/jem.170.3.1003.
Abstract
We have found cell surface expression of an E alpha molecule in recombinant and transgenic mouse strains lacking an E beta molecule. Flow cytometry has shown low level expression of E alpha in B10.RQB3 (I-AqEk alpha) and B10.RFB2 (I-AfEk alpha) mice. We have also found that B10.Q (H-2q) mice can express the Ek alpha transgene. Since these strains do not have functional E beta chains, we propose that the E alpha A beta hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E alpha/A beta molecule can function in vivo during thymic selection in the clonal deletion of two V beta TCR subsets, V beta 11 and V beta 6, which have been shown to interact with the intact I-E molecule.
摘要
我们在缺乏Eβ分子的重组和转基因小鼠品系中发现了Eα分子的细胞表面表达。流式细胞术显示，在B10.RQB3（I-AqEkα）和B10.RFB2（I-AfEkα）小鼠中，Eα有低水平表达。我们还发现B10.Q（H-2q）小鼠能够表达Ekα转基因。由于这些品系没有功能性的Eβ链，我们推测少量的EαAβ杂交二聚体得以形成，并且能够通过荧光激活细胞分选分析检测到。到目前为止，我们还无法通过细胞毒性或免疫沉淀鉴定出这些杂交分子。Eα/Aβ分子在体内胸腺选择过程中，对两个已被证明能与完整的I-E分子相互作用的VβTCR亚群（Vβ11和Vβ6）的克隆清除发挥作用。
相似文献
1
In vivo expression and function of hybrid Ia dimers (E alpha A beta) in recombinant and transgenic mice.重组和转基因小鼠中杂交Ia二聚体（EαAβ）的体内表达及功能
J Exp Med. 1989 Sep 1;170(3):1003-8. doi: 10.1084/jem.170.3.1003.
2
3
Role of Mls-1 locus and clonal deletion of T cells in susceptibility to collagen-induced arthritis in mice.Mls-1基因座及T细胞克隆性缺失在小鼠胶原诱导性关节炎易感性中的作用
J Immunol. 1991 Aug 15;147(4):1189-93.
4
Collagen-induced arthritis and TCRs in SWR and B10.Q mice expressing an Ek alpha transgene.表达Ekα转基因的SWR和B10.Q小鼠中的胶原诱导性关节炎及T细胞受体
J Immunol. 1994 Sep 15;153(6):2758-68.
5
Class I and class II MHC gene products differentially affect the fate of V beta 5 bearing thymocytes.I类和II类主要组织相容性复合体（MHC）基因产物对携带Vβ5的胸腺细胞的命运有不同影响。
J Mol Cell Immunol. 1990;4(5):269-79; discussion 279-80.
6
Role of the H-2 complex in induction of T helper cells in vivo. III. Contribution of the I-E subregion to restriction sites recognized by I-A/E-restricted T cells.H-2复合体在体内诱导T辅助细胞中的作用。III. I-E亚区对I-A/E限制性T细胞识别的限制位点的贡献。
J Exp Med. 1982 Feb 1;155(2):548-56. doi: 10.1084/jem.155.2.548.
7
Role of MHC, Mls and TCR in immune tolerance.主要组织相容性复合体（MHC）、次要淋巴细胞刺激（Mls）和T细胞受体（TCR）在免疫耐受中的作用。
J Immunogenet. 1989 Aug-Oct;16(4-5):257-61. doi: 10.1111/j.1744-313x.1989.tb00471.x.
8
Identification of I-E alpha genes in H-2 recombinant mouse strains by F1 complementation.通过F1互补鉴定H-2重组小鼠品系中的I-Eα基因。
Transplantation. 1987 Feb;43(2):297-301. doi: 10.1097/00007890-198702000-00026.
9
Immune response gene function correlates with the expression of an Ia antigen. II. A quantitative deficiency in Ae:E alpha complex expression causes a corresponding defect in antigen-presenting cell function.免疫反应基因功能与Ia抗原的表达相关。II. Ae:Eα复合体表达的定量缺陷导致抗原呈递细胞功能出现相应缺陷。
J Exp Med. 1982 Feb 1;155(2):508-23. doi: 10.1084/jem.155.2.508.
10
Weak positive selection of transgenic T cell receptor-bearing thymocytes: importance of major histocompatibility complex class II, T cell receptor and CD4 surface molecule densities.携带转基因T细胞受体的胸腺细胞的弱阳性选择：主要组织相容性复合体II类、T细胞受体和CD4表面分子密度的重要性。
Eur J Immunol. 1992 Mar;22(3):703-9. doi: 10.1002/eji.1830220313.
引用本文的文献
1
Characterization of an I-E-restricted, gp63-specific, CD4-T-cell clone from Leishmania major-resistant C3H mice that secretes type 2 cytokines and exacerbates infection with L. major.从对硕大利什曼原虫有抗性的C3H小鼠中分离出的一个I-E限制性、gp63特异性、分泌2型细胞因子并加剧硕大利什曼原虫感染的CD4 T细胞克隆的特性分析。
Infect Immun. 2004 Aug;72(8):4486-93. doi: 10.1128/IAI.72.8.4486-4493.2004.
2
Far upstream regions of class II MHC Ea are necessary for position-independent, copy-dependent expression of Ea transgene.II类MHC Ea的远上游区域对于Ea转基因的位置独立、拷贝依赖型表达是必需的。
Nucleic Acids Res. 1993 May 11;21(9):2065-72. doi: 10.1093/nar/21.9.2065.
3
Protective role of major histocompatibility complex class II Ebd transgene on collagen-induced arthritis.主要组织相容性复合体II类Ebd转基因对胶原诱导性关节炎的保护作用。
J Exp Med. 1994 Oct 1;180(4):1559-64. doi: 10.1084/jem.180.4.1559.
4
Alteration of the T-cell receptor repertoire in A.CA mice expressing an Ead transgene.表达Ead转基因的A.CA小鼠中T细胞受体库的改变。
Immunogenetics. 1991;33(3):178-83. doi: 10.1007/BF01719237.
5
Presentation of antigen by mixed isotype class II molecules in normal H-2d mice.正常H-2d小鼠中混合同种型II类分子对抗原的呈递。
J Exp Med. 1992 Jan 1;175(1):157-62. doi: 10.1084/jem.175.1.157.
6
The effect of excess beta-chain synthesis on cell-surface expression of allele-mismatched class II heterodimers in vivo.体内过量β链合成对等位基因错配的II类异二聚体细胞表面表达的影响。
Proc Natl Acad Sci U S A. 1990 Sep;87(18):7314-8. doi: 10.1073/pnas.87.18.7314.
本文引用的文献
1
Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.分泌针对小鼠H-2和Ia抗原的单克隆抗体的杂交瘤细胞系。
J Immunol. 1980 Feb;124(2):533-40.
2
Variable synthesis and expression of E alpha and Ae (E beta) Ia polypeptide chains in mice of different H-2 haplotypes.不同H-2单倍型小鼠中Eα和Ae（Eβ）Ia多肽链的可变合成与表达
Immunogenetics. 1981;12(3-4):321-37. doi: 10.1007/BF01561674.
3
Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes.d、k、p、r和u单倍型I-E抗原等位基因变异的生化记录。
Immunogenetics. 1982;16(5):393-405. doi: 10.1007/BF00372099.
4
Interaction between I region loci influences the expression of a cell surface Ia antigen.I区基因座之间的相互作用影响细胞表面Ia抗原的表达。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5404-8. doi: 10.1073/pnas.77.9.5404.
5
Membrane Ia expression and antigen-presenting accessory cell function of L cells transfected with class II major histocompatibility complex genes.转染了II类主要组织相容性复合体基因的L细胞的膜Ia表达及抗原呈递辅助细胞功能。
J Exp Med. 1984 Nov 1;160(5):1316-37. doi: 10.1084/jem.160.5.1316.
6
Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.DNA介导的基因转移后小鼠L细胞中I-Ak II类基因的表达。
Nature. 1983;305(5933):440-3. doi: 10.1038/305440a0.
7
Skin grafting.皮肤移植
Methods Enzymol. 1984;108:20-8. doi: 10.1016/s0076-6879(84)08070-8.
8
Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis.用于分析诱变特异性的定向诱变方法：应用于紫外线诱导的诱变
Proc Natl Acad Sci U S A. 1983 Jan;80(1):237-41. doi: 10.1073/pnas.80.1.237.
9
Free Ia E alpha chain expression in the E+ alpha : E- beta recombinant strain A.TFR5.E+α:E-β重组菌株A.TFR5中游离Ia Eα链的表达
Immunogenetics. 1985;22(6):523-32. doi: 10.1007/BF00430300.
10
T cell tolerance by clonal elimination in the thymus.胸腺中通过克隆清除实现的T细胞耐受性。
Cell. 1987 Apr 24;49(2):273-80. doi: 10.1016/0092-8674(87)90568-x.
Zotero 插件