Anderson G D, David C S
Department of Immunology, Mayo Clinic, Rochester, Minnesota 55905.
J Exp Med. 1989 Sep 1;170(3):1003-8. doi: 10.1084/jem.170.3.1003.
We have found cell surface expression of an E alpha molecule in recombinant and transgenic mouse strains lacking an E beta molecule. Flow cytometry has shown low level expression of E alpha in B10.RQB3 (I-AqEk alpha) and B10.RFB2 (I-AfEk alpha) mice. We have also found that B10.Q (H-2q) mice can express the Ek alpha transgene. Since these strains do not have functional E beta chains, we propose that the E alpha A beta hybrid dimers are formed in low numbers and can be picked up by FACS analysis. So far we have not been able to identify these hybrid molecules by cytotoxicity or immunoprecipitation. The E alpha/A beta molecule can function in vivo during thymic selection in the clonal deletion of two V beta TCR subsets, V beta 11 and V beta 6, which have been shown to interact with the intact I-E molecule.
我们在缺乏Eβ分子的重组和转基因小鼠品系中发现了Eα分子的细胞表面表达。流式细胞术显示,在B10.RQB3(I-AqEkα)和B10.RFB2(I-AfEkα)小鼠中,Eα有低水平表达。我们还发现B10.Q(H-2q)小鼠能够表达Ekα转基因。由于这些品系没有功能性的Eβ链,我们推测少量的EαAβ杂交二聚体得以形成,并且能够通过荧光激活细胞分选分析检测到。到目前为止,我们还无法通过细胞毒性或免疫沉淀鉴定出这些杂交分子。Eα/Aβ分子在体内胸腺选择过程中,对两个已被证明能与完整的I-E分子相互作用的VβTCR亚群(Vβ11和Vβ6)的克隆清除发挥作用。