俄罗斯莫斯科地区俄罗斯族个体细胞因子基因多态性的等位基因及基因型频率。

Allele and genotype frequencies of polymorphisms in cytokine genes in ethnic Russian individuals from Moscow, Russia.

作者信息

Shadrina Alexandra, Voronina Elena, Zolotukhin Igor, Filipenko Maxim

机构信息

Institute of Chemical Biology and Fundamental Medicine, Lavrentjeva Street, 8, Novosibirsk 630090, Russia.

出版信息

Hum Immunol. 2017 Feb;78(2):190-191. doi: 10.1016/j.humimm.2016.11.007. Epub 2016 Nov 23.

DOI:10.1016/j.humimm.2016.11.007

PMID:27888067

Abstract

Two hundred and twenty eight ethnic Russian individuals from Moscow, Russia, were genotyped at 14 single nucleotide polymorphisms CCL2 A-2578G; VEGFA C-2578A, G-634C, and C+936T; TNF G+419A and G-308A; IL1A G-889A; IL1RN T+1018C; IL6G-174C and G-572C; IFNG T+874A; IL1B C-511T; IL10 A+1082G; TGFB1 C-509T. Genotypes were determined using real-time polymerase chain reaction with TaqMan probes and polymerase chain reaction followed by melting analysis of dual-labeled probe. Genotype distribution was in accordance with Hardy-Weinberg equilibrium for all studied polymorphisms. Genotype data are available in the Allele Frequencies Net Database under identifier AFND 3367 and the population name "Russia Moscow Cytokine".

摘要

对来自俄罗斯莫斯科的228名俄罗斯族个体，就14个单核苷酸多态性进行了基因分型，这些多态性包括：CCL2基因A-2578G；VEGFA基因C-2578A、G-634C和C+936T；TNF基因G+419A和G-308A；IL1A基因G-889A；IL1RN基因T+1018C；IL6基因G-174C和G-572C；IFNG基因T+874A；IL1B基因C-511T；IL10基因A+1082G；TGFB1基因C-509T。使用带有TaqMan探针的实时聚合酶链反应以及聚合酶链反应随后进行双标记探针熔解分析来确定基因型。对于所有研究的多态性，基因型分布均符合哈迪-温伯格平衡。基因型数据可在等位基因频率网络数据库中获取，标识符为AFND 3367，群体名称为“俄罗斯莫斯科细胞因子”。