Muhlach W L, Pollack E D
Department of Biological Sciences, University of Illinois, Chicago 60608.
Int J Dev Neurosci. 1989;7(4):383-7. doi: 10.1016/0736-5748(89)90059-2.
Choline acetyltransferase (ChAT) activity in developing spinal cord explants in vitro is shown to be dependent on the presence of co-cultured immature limb tissue. Frog tadpole spinal cord explants grown on collagen or polylysine expressed stage-appropriate levels of ChAT activity only when in the presence of the limb mesenchyme target. Neither skeletal muscle nor polylysine, both of which enhance neurite growth accompanied by increases in cord protein, were capable of maintaining the level of ChAT activity characteristic of these spinal cords in vivo. The results demonstrate that developmentally significant levels of ChAT can be maintained in vitro under appropriate conditions that may act in part through the maintenance of cholinergic motor neurons.
体外培养的发育中的脊髓外植体中的胆碱乙酰转移酶（ChAT）活性被证明依赖于共培养的未成熟肢体组织的存在。仅当存在肢体间充质靶标时，在胶原蛋白或聚赖氨酸上生长的青蛙蝌蚪脊髓外植体才表达与阶段相适应的ChAT活性水平。骨骼肌和聚赖氨酸都能促进神经突生长并伴随着脊髓蛋白增加，但两者都不能维持这些脊髓在体内所特有的ChAT活性水平。结果表明，在适当条件下，体外可维持具有发育意义水平的ChAT，这些条件可能部分通过维持胆碱能运动神经元起作用。
