Wu Bin, Jiang Hongli, He Quan, Wang Meng, Xue Jinhong, Liu Hua, Shi Kehui, Wei Meng, Liang Shanshan, Zhang Liwen
Dialysis Centre of First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, China.
Nephron. 2017;135(2):156-166. doi: 10.1159/000452453. Epub 2016 Nov 26.
BACKGROUND/AIM: Chronic kidney disease is accompanied by changes in the gut microbiome and by an increase in the number of gut pathogenic bacteria. The aim of this study was to investigate the difference of the faecal metabolic profiles in rats with uremia, and to determine whether the altered metabolites in the rats with uremia can be restored by Lactobacillus.
Thirty rats were randomly divided into 3 groups: sham, uremia and uremia + probiotic (UP) groups. The rats in uremia and UP groups were prepared through surgical renal mass 5/6 ablation. The rats in the UP group received Lactobacillus LB (1 ml, 109 CFU/ml) through gavage every day for 4 weeks. The rats were fed with a standard diet. Faecal samples were analysed through ultra performance liquid chromatography/mass spectrometry. Statistical analyses were performed using MetaboAnalyst and MATLAB.
A total of 99, 324 and 177 significantly different ion peaks were selected between sham and uremia groups; sham and UP groups; and uremia and UP groups, respectively. In the 3 groups, 35 significantly altered metabolites were identified; of the 35 metabolites, 27 initially increased and then decreased; by contrast, 8 metabolites initially decreased and then increased. The 35 metabolites were subjected to pathway analysis in MetaboAnalyst.
Faecal metabolites were significantly altered in rats with uremia; these changes were partially reversed by Lactobacillus.
背景/目的：慢性肾脏病伴有肠道微生物群的变化以及肠道致病菌数量的增加。本研究旨在探讨尿毒症大鼠粪便代谢谱的差异，并确定尿毒症大鼠体内改变的代谢产物是否能通过乳酸杆菌恢复。
30只大鼠随机分为3组：假手术组、尿毒症组和尿毒症+益生菌（UP）组。尿毒症组和UP组大鼠通过手术切除5/6的肾脏组织制备而成。UP组大鼠每天经口灌胃给予乳酸杆菌LB（1 ml，109 CFU/ml），持续4周。大鼠喂食标准饮食。粪便样本通过超高效液相色谱/质谱分析。使用MetaboAnalyst和MATLAB进行统计分析。
假手术组与尿毒症组、假手术组与UP组、尿毒症组与UP组之间分别共筛选出99、324和177个显著不同的离子峰。在这3组中，鉴定出35种显著改变的代谢产物；在这35种代谢产物中，27种最初升高然后降低；相比之下，8种代谢产物最初降低然后升高。对这35种代谢产物在MetaboAnalyst中进行通路分析。
尿毒症大鼠的粪便代谢产物发生了显著改变；这些变化部分被乳酸杆菌逆转。
