Bando Y, Tanouchi Y, Fukuyado K, Matsuda S, Mimura Y
Nippon Ganka Gakkai Zasshi. 1989 Mar;93(3):369-74.
The dynamics of leukocytes, complement and tumor necrosis factor (TNF) were studied in endotoxin induced uveitis (EIU) in rats. Endotoxin was administered to footpads and the time courses of the following were examined: 1) the number of leukocytes in peripheral blood 2) the number of leukocytes and protein concentration of aqueous humor 3) complement (CH50, C1, C3, C5) in serum and aqueous humor 4) TNF in serum 5) histological changes in the eyes, lungs, liver, and skin. The number of leukocytes in peripheral blood decreased to one third that of controls during three to six hours after endotoxin administration, but increased thereafter along with the number of leukocytes and protein concentration in aqueous humor. More leukocytes were observed than in controls in the small vessels of the iris, lung, liver and skin, some of which attached to the vascular endothelium. Serum C3 increased and serum C5 transiently decreased after endotoxin administration. Serum TNF reached a peak (3500 IU/ml) at 1.5 hours and rapidly decreased subsequently. It is suggested that the adhesion of leukocytes to vascular endothelium and the activation of complement system may play important roles in the development of EIU.
研究了大鼠内毒素诱导性葡萄膜炎（EIU）中白细胞、补体和肿瘤坏死因子（TNF）的动态变化。将内毒素注射到足垫，并检测以下各项的时间进程：1）外周血中白细胞数量；2）房水中白细胞数量和蛋白质浓度；3）血清和房水中的补体（CH50、C1、C3、C5）；4）血清中的TNF；5）眼、肺、肝和皮肤的组织学变化。内毒素注射后三至六小时，外周血中白细胞数量降至对照组的三分之一，但此后随着房水中白细胞数量和蛋白质浓度的增加而增加。在虹膜、肺、肝和皮肤的小血管中观察到的白细胞比对照组更多，其中一些附着在血管内皮上。内毒素注射后血清C3升高，血清C5短暂降低。血清TNF在1.5小时达到峰值（3500 IU/ml），随后迅速下降。提示白细胞与血管内皮的黏附以及补体系统的激活可能在EIU的发生发展中起重要作用。
