De Francesco A, Costa N, García M L
Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET - UNLP, calles 47 y 115, 1900, La Plata, Buenos Aires, Argentina.
Estación Experimental Agropecuaria, INTA Concordia, Ruta Provincial 22 y vías del Ferrocarril, 3200, Concordia, Entre Ríos, Argentina.
Transgenic Res. 2017 Apr;26(2):225-235. doi: 10.1007/s11248-016-0001-2. Epub 2016 Nov 28.
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.
柑橘速衰病毒（CPsV）是速衰病的致病因子，速衰病是一种严重且广泛传播的柑橘病害。已经描述了速衰病的两种症状类型，即PsA和PsB。PsB是最具侵袭性且蔓延性最强的类型。此前，我们获得了用源自CPsV外壳蛋白基因（ihpCP）的发夹结构转化的菠萝甜橙植株。其中一些植株对CPsV 90-1-1具有抗性，CPsV 90-1-1是一种与转基因同源的PsA分离株。在本研究中，我们发现ihpCP转基因在ihpCP-10和-15株系中的表达以及siRNA的产生随时间和繁殖过程保持稳定。特别是，ihpCP-15株系已经连续2年多具有抗性，即使在再次接种后也是如此。ihpCP植株对导致严重PsB症状的异源CPsV分离株也具有抗性。ihpCP-15株系表现出完全抗性，而ihpCP-10株系对该病毒具有耐受性，不过表现出不同的抗性表现，在PsB症状上出现延迟和减轻。这些株系有望用于一种旨在根除速衰病的生物技术产品。
