Kluin-Nelemans H C, Jansen J H
Laboratory of Experimental Hematology, University Hospital, Leiden, The Netherlands.
Leukemia. 1989 Oct;3(10):715-7.
Malignant B cells, derived from hairy cell leukemia (HCL), non-Hodgkin's lymphoma (NHL) or prolymphocytic leukemia were stimulated with mitogens and interleukin 2 after careful depletion of contaminating T cells resulting in final residual percentages of less than 0.1. No proliferation was found as measured by 3H-thymidine incorporation. Subsequently, to the non-T B cell cultures very small amounts of autologous or allogeneic T cells were added, ending up in final concentrations of 0.1, 0.5, 1, or 2% T cells. It appeared that a marked proliferation occurred which had in various coculture combinations to be ascribed to the T cells alone. Moreover, most HCL and other B cell NHL additionally stimulated the T cells, resulting in a further increase in proliferation. We conclude that 3H-thymidine incorporation by malignant B cells such as HCL and B-NHL stimulated with mitogens and IL-2 will in most cases wrongly be attributed to proliferation by the B cells themselves, and instead has to be ascribed to contaminating T cells.
来自毛细胞白血病（HCL）、非霍奇金淋巴瘤（NHL）或原淋巴细胞白血病的恶性B细胞，在仔细去除污染的T细胞后，用丝裂原和白细胞介素2进行刺激，最终残余百分比小于0.1%。通过3H-胸苷掺入法检测未发现增殖。随后，向非T B细胞培养物中加入极少量的自体或同种异体T细胞，最终T细胞浓度达到0.1%、0.5%、1%或2%。结果显示出现了明显的增殖，在各种共培养组合中，这种增殖只能归因于单独的T细胞。此外，大多数HCL和其他B细胞NHL还会进一步刺激T细胞，导致增殖进一步增加。我们得出结论，在大多数情况下，用丝裂原和白细胞介素2刺激的恶性B细胞（如HCL和B-NHL）的3H-胸苷掺入错误地归因于B细胞自身的增殖，而实际上应归因于污染的T细胞。
