Department of Pharmacology, Guangxi Medical University, Nanning, China.
Guangxi University Library, Guangxi University, Nanning, China.
Biomed Pharmacother. 2017 Jan;85:611-619. doi: 10.1016/j.biopha.2016.11.071. Epub 2016 Nov 25.
The anti-fibrotic mechanism of combination therapy with taurine, epigallocatechin gallate and genistein was studied from the perspective of serum proteomics in our previous work. In order to further investigate and systematically analyse other possible therapeutic mechanism of combination therapy against liver fibrosis, isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis was applied to study the protein profile changes in liver tissue of carbon tetrachloride-induced liver fibrosis rats after combination therapy. A total of 115 differentially expressed proteins containing 84 up-regulated and 31 down-regulated proteins in response to combination therapy were identified. Three differentially expressed proteins (Txn1, Ctsd and Cdk4) involved in antioxidant defense system and the activation and proliferation of hepatic stellate cell were selected for further validation by western blot and real-time PCR analysis. Our study highlight the importance of differentially expressed proteins Txn1, Ctsd and Cdk4 against liver fibrosis, which may provide a more precise and comprehensive perspective for clarifying the roles of combination therapy as a potential agent for treatment of liver fibrosis.
我们之前的工作从血清蛋白质组学的角度研究了牛磺酸、表没食子儿茶素没食子酸酯和染料木黄酮联合治疗的抗纤维化机制。为了进一步研究和系统分析联合治疗抗肝纤维化的其他可能治疗机制，我们应用等重标记相对和绝对定量（iTRAQ）蛋白质组学分析方法研究了联合治疗后四氯化碳诱导的肝纤维化大鼠肝组织中的蛋白质谱变化。共鉴定出 115 种差异表达蛋白，其中 84 种上调，31 种下调。选择三个差异表达蛋白（Txn1、Ctsd 和 Cdk4），它们涉及抗氧化防御系统以及肝星状细胞的激活和增殖，通过 Western blot 和实时 PCR 分析进行进一步验证。我们的研究强调了差异表达蛋白 Txn1、Ctsd 和 Cdk4 对肝纤维化的重要性，这可能为阐明联合治疗作为肝纤维化治疗潜在药物的作用提供更精确和全面的视角。
