Liu Yun, Hu Xu, Xia Daokui, Zhang Songlin
Department of Cardiothoracic Surgery, The First College of Clinical Medical Science, China Three Gorges University, Yichang, Hubei 443000, P.R. China; Department of Cardiothoracic Surgery, Yichang Central People's Hospital, Yichang, Hubei 443000, P.R. China.
Oncol Lett. 2016 Nov;12(5):4181-4186. doi: 10.3892/ol.2016.5198. Epub 2016 Sep 28.
The expression of microRNA-181b (miR-181b) has been investigated in various human cancers. However, the expression and functions of miR-181b in non-small cell lung cancer (NSCLC) are yet to be studied. In the present study, miR-181b expression in NSCLC tissues and cell lines was analyzed by quantitative polymerase chain reaction, and was shown to be recurrently downregulated. Following transfection of the H23 and H522 NSCLC cells lines with miR-181b, cell migration and cell invasion assays were performed to evaluate the effect of miR-181b overexpression on the cell motility. It was demonstrated that overexpression of miR-181b inhibited the migration and invasion of NSCLC cells. Subsequently, bioinformatics analysis, western blotting and luciferase reporter assays were conducted to investigate the mechanism underlying the miR-181b-mediated inhibition of NSCLC cell motility. It was found that miR-181b directly targeted high-mobility group box-1 (HMGB1) in NSCLC cells. These results reveal a novel therapeutic target, the miR-181b/HMGB1 axis, in NSCLC. Treatment approaches targeting this axis will be beneficial to prevent NSCLC from becoming invasive.
微小RNA-181b（miR-181b）的表达已在多种人类癌症中得到研究。然而，miR-181b在非小细胞肺癌（NSCLC）中的表达和功能尚未见报道。在本研究中，采用定量聚合酶链反应分析了NSCLC组织和细胞系中miR-181b的表达，结果显示其表达经常下调。在用miR-181b转染H23和H522 NSCLC细胞系后，进行细胞迁移和侵袭试验以评估miR-181b过表达对细胞运动性的影响。结果表明，miR-181b过表达抑制了NSCLC细胞的迁移和侵袭。随后，通过生物信息学分析、蛋白质印迹法和荧光素酶报告基因检测来研究miR-181b介导的NSCLC细胞运动性抑制的机制。研究发现，miR-181b在NSCLC细胞中直接靶向高迁移率族蛋白B1（HMGB1）。这些结果揭示了NSCLC中的一个新的治疗靶点，即miR-181b/HMGB1轴。针对该轴的治疗方法将有助于预防NSCLC的侵袭。
