Moreno-Hagelsieb Gabriel
Department of Biology, Wilfrid Laurier University, 75 University Ave W, Waterloo, ON, Canada, N2L 3C5.
Methods Mol Biol. 2017;1526:41-63. doi: 10.1007/978-1-4939-6613-4_3.
Predicting functional associations using the Gene Neighbor Method depends on the simple idea that if genes are conserved next to each other in evolutionarily distant prokaryotes they might belong to a polycistronic transcription unit. The procedure presented in this chapter starts with the organization of the genes within genomes into pairs of adjacent genes. Then, the pairs of adjacent genes in a genome of interest are mapped to their corresponding orthologs in other, informative, genomes. The final step is to verify if the mapped orthologs are also pairs of adjacent genes in the informative genomes.
使用基因邻接方法预测功能关联依赖于一个简单的想法，即如果基因在进化距离较远的原核生物中彼此相邻保守，那么它们可能属于一个多顺反子转录单元。本章介绍的方法首先将基因组中的基因组织成相邻基因对。然后，将目标基因组中的相邻基因对映射到其他信息丰富的基因组中的相应直系同源基因。最后一步是验证映射的直系同源基因在信息丰富的基因组中是否也是相邻基因对。
