Takatsuji K, Tohyama M
Osaka Prefectural College of Nursing, Japan.
J Chem Neuroanat. 1989 Jan-Feb;2(1):19-27.
Geniculo-geniculate projections of immunoreactive neurons for gamma-aminobutyric acid (GABA), leucine-enkephalin (ENK), neuropeptide Y (NPY) and substance P (SP) in the intergeniculate leaflet (IGL) and ventral lateral geniculate nucleus (VLG) of the rat were examined by using a combination of retrograde tracing method and immunocytochemistry. After injection of the fast blue (FB) dye into the IGL and VLG, many ENK immunoreactive neurons, and some NPY immunoreactive neurons were labelled by FB dye in the contralateral IGL. However, GABA and SP immunoreactive neurons in the IGL and VLG were not labelled retrogradely by FB dye. A unilateral electrical lesion of the IGL and VLG caused a reduction of the ENK and NPY immunoreactive fibres in the contralateral IGL and VLG. These findings suggested that ENK is one major component of the neuroactive substances in the geniculo-geniculate projection, and NPY also contributes partly to this projection. Furthermore, the unilateral destruction of the IGL and VLG showed a marked loss of the NPY immunoreactive fibres and a slight loss of the ENK immunoreactive fibres in the bilateral suprachiasmatic nuclei.
采用逆行追踪法与免疫细胞化学相结合的方法，研究了大鼠间膝小叶（IGL）和腹外侧膝状核（VLG）中γ-氨基丁酸（GABA）、亮氨酸脑啡肽（ENK）、神经肽Y（NPY）和P物质（SP）免疫反应性神经元的膝-膝投射。将快蓝（FB）染料注入IGL和VLG后，对侧IGL中有许多ENK免疫反应性神经元以及一些NPY免疫反应性神经元被FB染料标记。然而，IGL和VLG中的GABA和SP免疫反应性神经元未被FB染料逆行标记。IGL和VLG的单侧电损伤导致对侧IGL和VLG中ENK和NPY免疫反应性纤维减少。这些发现表明，ENK是膝-膝投射中神经活性物质的一个主要成分，NPY也部分参与了这一投射。此外，IGL和VLG的单侧破坏显示双侧视交叉上核中NPY免疫反应性纤维明显减少，ENK免疫反应性纤维略有减少。
